Department of Nutritional Toxicology, Institute of Nutrition, Friedrich Schiller University Jena, Germany.
Free Radic Biol Med. 2012 Nov 1;53(9):1760-9. doi: 10.1016/j.freeradbiomed.2012.08.591. Epub 2012 Sep 1.
In the current literature, the lysosomal system is considered to be involved in the intracellular formation and accumulation of lipofuscin, a highly oxidized and covalently cross-linked aggregate of proteins that fills the lysosomal volume during aging. In contrast, our experimental results presented here suggest that both the autophagosomes and the lysosomal system are not mandatory for the formation of lipofuscin, since that material accumulates in the cytosolic volume if autophagy or lysosomal activity is inhibited. However, such an inhibition is accompanied by an enhanced toxicity of the formed protein aggregates. Furthermore, it could be proven that macroautophagy is responsible for the uptake of lipofuscin into the lysosomes.
在当前的文献中，溶酶体系统被认为参与了脂褐素的细胞内形成和积累，脂褐素是一种高度氧化和共价交联的蛋白质聚集体，在衰老过程中充满了溶酶体体积。相比之下，我们在这里提出的实验结果表明，自噬体和溶酶体系统对于脂褐素的形成都不是必需的，因为如果自噬或溶酶体活性被抑制，这种物质会在胞质体积中积累。然而，这种抑制伴随着形成的蛋白质聚集体毒性的增强。此外，还可以证明巨自噬负责将脂褐素摄取到溶酶体中。
