Girardie J, Girardie A, Huet J C, Pernollet J C
Laboratoire de Neuroendocrinologie, UA CNRS 1138, Université de Bordeaux I, Talence, France.
FEBS Lett. 1989 Mar 13;245(1-2):4-8. doi: 10.1016/0014-5793(89)80179-6.
Neuroparsins A and B were isolated from the nervous part of the corpus cardiaca of Locusta migratoria via a two-step purification procedure. Both consist of two polypeptide chains linked by disulfide bridges. The N-terminal sequence of both native neuroparsins was determined: the N-terminal end of neuroparsin B was unique while that of neuroparsin A showed three different sequences. These sequences were that of neuroparsin B and two others having five and two extra N-terminal residues. Neuroparsin B was found as a homodimer and the complete sequence of the monomer, determined from peptide fragments generated by treatment with cyanogen bromide and endoprotease Glu-C, comprises 78 residues.
