Department of Biology, Plant Molecular and Cellular Biology Program, Genetics Institute, University of Florida, Gainesville, FL 32610, USA.
Mol Cell Proteomics. 2012 Dec;11(12):1622-30. doi: 10.1074/mcp.R112.021550. Epub 2012 Sep 16.
Multicellular organisms such as plants contain different types of cells with specialized functions. Analyzing the protein characteristics of each type of cell will not only reveal specific cell functions, but also enhance understanding of how an organism works. Most plant proteomics studies have focused on using tissues and organs containing a mixture of different cells. Recent single-cell-type proteomics efforts on pollen grains, guard cells, mesophyll cells, root hairs, and trichomes have shown utility. We expect that high resolution proteomic analyses will reveal novel functions in single cells. This review provides an overview of recent developments in plant single-cell-type proteomics. We discuss application of the approach for understanding important cell functions, and we consider the technical challenges of extending the approach to all plant cell types. Finally, we consider the integration of single-cell-type proteomics with transcriptomics and metabolomics with the goal of providing a holistic understanding of plant function.
多细胞生物（如植物）包含具有特定功能的不同类型的细胞。分析每种类型细胞的蛋白质特征不仅可以揭示特定的细胞功能，还可以增进对生物体如何工作的理解。大多数植物蛋白质组学研究都集中在使用含有不同细胞混合物的组织和器官上。最近对花粉粒、保卫细胞、叶肉细胞、根毛和表皮毛进行的单细胞型蛋白质组学研究表明其具有实用性。我们预计高分辨率蛋白质组学分析将揭示单细胞中的新功能。本文综述了植物单细胞型蛋白质组学的最新进展。我们讨论了该方法在理解重要细胞功能方面的应用，并考虑了将该方法扩展到所有植物细胞类型的技术挑战。最后，我们考虑将单细胞型蛋白质组学与转录组学和代谢组学相结合，以全面了解植物功能。
