Papp Hajnalka, Al-Mutairi Laila Zaar, Chehadeh Wassim, Farkas Szilvia L, Lengyel György, Jakab Ferenc, Martella Vito, Szűcs György, Bányai Krisztián
Hungarian Academy of Sciences, Institute for Veterinary Medical Research, Budapest, Hungary.
Acta Microbiol Immunol Hung. 2012 Sep;59(3):411-21. doi: 10.1556/AMicr.59.2012.3.11.
In this study a Kuwaiti camel rotavirus strain, RVA/Camel-wt/KUW/s21/2010/G10P[15], is characterized by sequencing and phylogenetic analysis. The strain had multiple genes with high nucleotide sequence similarities to ovine and bovine strains (VP2, ≤ 96%; NSP2 and NSP5, ≤ 97%, NSP3, ≤ 94%), or, to porcine strains (VP1, ≤ 89%). Other genes had moderate sequence similarities (VP4, ≤ 87%; VP6, ≤ 81%; VP7, ≤ 82%) with reference strains from ruminants. The NSP4 gene shared limited sequence identity (≤ 71%) with other mammalian and avian rotavirus NSP4 types, and was designated a novel genotype, E15. This study demonstrates genetic diversity in the outer capsid and some backbone genes of an old-world camelid rotavirus strain and uncovers its common evolutionary roots with strains from other ruminants.
在本研究中，通过测序和系统发育分析对一株科威特骆驼轮状病毒毒株RVA/Camel-wt/KUW/s21/2010/G10P[15]进行了特征分析。该毒株的多个基因与绵羊和牛的毒株具有较高的核苷酸序列相似性（VP2，≤96%；NSP2和NSP5，≤97%，NSP3，≤94%），或者与猪的毒株具有较高的核苷酸序列相似性（VP1，≤89%）。其他基因与反刍动物参考毒株具有中等程度的序列相似性（VP4，≤87%；VP6，≤81%；VP7，≤82%）。NSP4基因与其他哺乳动物和禽轮状病毒NSP4型的序列同一性有限（≤71%），并被指定为一种新的基因型E15。本研究证明了旧大陆骆驼科动物轮状病毒毒株外衣壳和一些主干基因的遗传多样性，并揭示了其与其他反刍动物毒株的共同进化根源。
