Centre de Recherche Public - Gabriel Lippmann, Department EVA, 41, rue du Brill, L-4422 Belvaux, Luxembourg.
Plant Physiol Biochem. 2012 Nov;60:180-9. doi: 10.1016/j.plaphy.2012.08.003. Epub 2012 Aug 31.
Potato is one of the most important staple food in the world because it is a good source of vitamin C, vitamin B6 but also an interesting source of minerals including mainly potassium, but also magnesium, phosphorus, manganese, zinc and iron to a lesser extent. The lack of iron constitutes the main form of micronutrient deficiency in the world, namely iron deficiency anemia, which strongly affects pregnant women and children from developing countries. Iron biofortification of major staple food such as potato is thus a crucial issue for populations from these countries. To better understand mechanisms leading to iron accumulation in potato, we followed in an in vitro culture experiment, by qPCR, in the cultivar Désirée, the influence of media iron content on the expression of genes related to iron uptake, transport and homeostasis. As expected, plantlets grown in a low iron medium (1 mg L(-1) FeNaEDTA) displayed a decreased iron content, a strong induction of iron deficiency-related genes and a decreased expression of ferritins. Inversely, plantlets grown in a high iron medium (120 mg L(-1) FeNaEDTA) strongly accumulated iron in roots; however, no significant change in the expression of our set of genes was observed compared to control (40 mg L(-1) FeNaEDTA).
土豆是世界上最重要的主食之一，因为它是维生素 C 和维生素 B6 的良好来源，也是矿物质的有趣来源，包括主要的钾，以及镁、磷、锰、锌和铁，但其含量较少。缺铁是世界上主要的微量营养素缺乏形式，即缺铁性贫血，它严重影响发展中国家的孕妇和儿童。因此，对这些国家的人口来说，将主要主食如土豆进行铁生物强化是一个至关重要的问题。为了更好地理解导致土豆中铁积累的机制，我们在体外培养实验中通过 qPCR 研究了在品种 Desiree 中，培养基中铁含量对与铁摄取、运输和稳态相关的基因表达的影响。正如预期的那样，在低铁培养基（1 mg/L FeNaEDTA）中生长的植物苗显示出铁含量降低，缺铁相关基因强烈诱导，铁蛋白表达降低。相反，在高铁培养基（120 mg/L FeNaEDTA）中生长的植物苗在根部强烈积累铁；然而，与对照（40 mg/L FeNaEDTA）相比，我们所研究的这组基因的表达没有明显变化。
