Department of Anatomy, The Catholic University of Korea, Seoul, Korea.
J Histochem Cytochem. 2013 Jan;61(1):31-44. doi: 10.1369/0022155412462975. Epub 2012 Sep 13.
We investigated the spatiotemporal expression of vascular endothelial growth factor receptor-3 (VEGFR-3) in the spinal cord of Lewis rats with experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis. VEGFR-3 mRNA and protein were constitutively expressed in gray matter neurons and in a few white matter astrocytes. Induction of VEGFR-3 occurred predominantly in perivascular infiltrated macrophages in the spinal cord white matter during the inductive phase of EAE. VEGFR-3 expression was also induced in activated microglial cells in the gray and white matter, mainly in the peak phase. In addition, reactive astrocytes in the white matter, but not in the gray matter, expressed VEGFR-3 as disease severity increased. These data suggest that VEGFR-3 is involved in the recruitment of monocytic macrophages and in glial reactions during EAE.
我们研究了血管内皮生长因子受体-3（VEGFR-3）在实验性自身免疫性脑脊髓炎（EAE）大鼠脊髓中的时空表达，EAE 是多发性硬化症的动物模型。VEGFR-3mRNA 和蛋白在灰质神经元和少数白质星形胶质细胞中持续表达。在 EAE 的诱导期，VEGFR-3 的诱导主要发生在脊髓白质血管周围浸润的巨噬细胞中。VEGFR-3 的表达也在灰质和白质中激活的小胶质细胞中诱导，主要在高峰期。此外，随着疾病严重程度的增加，反应性星形胶质细胞在白质中表达 VEGFR-3，但不在灰质中表达。这些数据表明，VEGFR-3 参与了 EAE 期间单核巨噬细胞的募集和神经胶质反应。
