School of Life and Basic Sciences, Sichuan Agricultural University, Yaan, Sichuan, People's Republic of China.
Funct Integr Genomics. 2012 Nov;12(4):683-91. doi: 10.1007/s10142-012-0290-3. Epub 2012 Sep 15.
Maize (Zea mays ssp. mays L.) is an important model organism for fundamental research in the agro-biotechnology field. Aldehydes were generated in response to a suite of environmental stresses that perturb metabolism including salinity, dehydration, desiccation, and cold and heat shock. Many biologically important aldehydes are metabolized by the superfamily of NAD(P)(+)-dependent aldehyde dehydrogenases. Here, starting from the database of Z. mays, we identified 28 aldehyde dehydrogenase (ALDH) genes and 48 transcripts by the in silico cloning method using the ALDH-conserved domain amino acid sequence of Arabidopsis and rice as a probe. Phylogenetic analysis shows that all 28 members of the ALDH gene families were classified to ten distinct subfamilies. Microarray data and quantitative real-time PCR analysis reveal that ZmALDH9, ZmALDH13, and ZmALDH17 genes involve the function of drought stress, acid tolerance, and pathogens infection. These results suggested that these three ZmALDH genes might be potentially useful in maize genetic improvement.
玉米（Zea mays ssp. mays L.）是农业生物技术领域基础研究的重要模式生物。醛类物质是响应一系列环境胁迫而产生的，这些胁迫会扰乱代谢，包括盐度、脱水、干燥以及冷和热冲击。许多具有重要生物学意义的醛类物质都被 NAD(P)(+) 依赖的醛脱氢酶超家族代谢。在这里，我们使用拟南芥和水稻的 ALDH 保守结构域氨基酸序列作为探针，通过计算机克隆方法，从玉米数据库中鉴定出 28 个醛脱氢酶 (ALDH) 基因和 48 个转录本。系统发育分析表明，所有 28 个 ALDH 基因家族成员都被分为十个不同的亚家族。微阵列数据和定量实时 PCR 分析表明，ZmALDH9、ZmALDH13 和 ZmALDH17 基因参与干旱胁迫、耐酸和病原体感染的功能。这些结果表明，这三个 ZmALDH 基因可能在玉米遗传改良中具有潜在的应用价值。
