Biometris, Wageningen University and Research Centre, P.O. Box 100, 6700 AC, Wageningen, The Netherlands.
Theor Appl Genet. 2013 Feb;126(2):289-305. doi: 10.1007/s00122-012-1971-y. Epub 2012 Sep 15.
Definition of clear criteria for evaluation of the quality of core collections is a prerequisite for selecting high-quality cores. However, a critical examination of the different methods used in literature, for evaluating the quality of core collections, shows that there are no clear guidelines on the choices of quality evaluation criteria and as a result, inappropriate analyses are sometimes made leading to false conclusions being drawn regarding the quality of core collections and the methods to select such core collections. The choice of criteria for evaluating core collections appears to be based mainly on the fact that those criteria have been used in earlier publications rather than on the actual objectives of the core collection. In this study, we provide insight into different criteria used for evaluating core collections. We also discussed different types of core collections and related each type of core collection to their respective evaluation criteria. Two new criteria based on genetic distance are introduced. The consequences of the different evaluation criteria are illustrated using simulated and experimental data. We strongly recommend the use of the distance-based criteria since they not only allow the simultaneous evaluation of all variables describing the accessions, but they also provide intuitive and interpretable criteria, as compared with the univariate criteria generally used for the evaluation of core collections. Our findings will provide genebank curators and researchers with possibilities to make informed choices when creating, comparing and using core collections.
定义明确的核心种质评价标准是选择高质量核心种质的前提。然而，对文献中用于评估核心种质质量的不同方法进行批判性检查表明，对于质量评估标准的选择没有明确的指导方针，因此有时会进行不合适的分析，导致对核心种质质量和选择此类核心种质的方法得出错误的结论。评估核心种质的标准的选择似乎主要基于这些标准已在早期出版物中使用这一事实，而不是基于核心种质的实际目标。在本研究中，我们深入了解了用于评估核心种质的不同标准。我们还讨论了不同类型的核心种质，并将每种类型的核心种质与其各自的评估标准相关联。引入了两个基于遗传距离的新标准。使用模拟和实验数据说明了不同评估标准的后果。我们强烈建议使用基于距离的标准，因为它们不仅允许同时评估描述访问的所有变量，而且与通常用于评估核心种质的单变量标准相比，它们还提供了直观和可解释的标准。我们的研究结果将为基因库管理者和研究人员在创建、比较和使用核心种质时提供明智的选择。
