School of Life Sciences, University of Nevada at Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154, USA.
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1824-1833. doi: 10.1099/ijs.0.044453-0. Epub 2012 Sep 14.
A magnetotactic bacterium, designated strain MV-1(T), was isolated from sulfide-rich sediments in a salt marsh near Boston, MA, USA. Cells of strain MV-1(T) were Gram-negative, and vibrioid to helicoid in morphology. Cells were motile by means of a single polar flagellum. The cells appeared to display a transitional state between axial and polar magnetotaxis: cells swam in both directions, but generally had longer excursions in one direction than the other. Cells possessed a single chain of magnetosomes containing truncated hexaoctahedral crystals of magnetite, positioned along the long axis of the cell. Strain MV-1(T) was a microaerophile that was also capable of anaerobic growth on some nitrogen oxides. Salinities greater than 10 % seawater were required for growth. Strain MV-1(T) exhibited chemolithoautotrophic growth on thiosulfate and sulfide with oxygen as the terminal electron acceptor (microaerobic growth) and on thiosulfate using nitrous oxide (N2O) as the terminal electron acceptor (anaerobic growth). Chemo-organoautotrophic and methylotrophic growth was supported by formate under microaerobic conditions. Autotrophic growth occurred via the Calvin-Benson-Bassham cycle. Chemo-organoheterotrophic growth was supported by various organic acids and amino acids, under microaerobic and anaerobic conditions. Optimal growth occurred at pH 7.0 and 26-28 °C. The genome of strain MV-1(T) consisted of a single, circular chromosome, about 3.7 Mb in size, with a G+C content of 52.9-53.5 mol%.Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MV-1(T) belongs to the family Rhodospirillaceae within the Alphaproteobacteria, but is not closely related to the genus Magnetospirillum. The name Magnetovibrio blakemorei gen. nov., sp. nov. is proposed for strain MV-1(T). The type strain of Magnetovibrio blakemorei is MV-1(T) ( = ATCC BAA-1436(T) = DSM 18854(T)).
一种趋磁细菌，命名为 MV-1(T) 菌株，从美国马萨诸塞州波士顿附近的一个盐沼富含硫化物的沉积物中分离得到。MV-1(T) 菌株的细胞呈革兰氏阴性，形态为弧菌到螺旋菌。细胞通过单个极鞭毛运动。细胞似乎表现出轴向和极趋磁之间的过渡状态：细胞向两个方向游动，但通常在一个方向上的游动距离比另一个方向长。细胞含有一条磁小体链，其中含有截短的六方八面体磁铁矿晶体，沿着细胞的长轴排列。MV-1(T) 菌株是一种微好氧菌，也能够在一些氮氧化物上进行厌氧生长。需要大于 10%海水的盐度才能生长。MV-1(T) 菌株在硫代硫酸盐和硫化物上进行化能自养生长，以氧气为末端电子受体（微好氧生长），并在硫代硫酸盐上使用氧化亚氮（N2O）作为末端电子受体（厌氧生长）。在微好氧条件下，通过甲酸盐支持化能有机自养和甲基营养生长。自养生长通过卡尔文-本森-巴斯汉姆循环进行。在微好氧和厌氧条件下，通过各种有机酸和氨基酸支持化能有机异养生长。最佳生长发生在 pH 值为 7.0 和 26-28°C。MV-1(T) 菌株的基因组由一个大小约为 3.7 Mb 的单一圆形染色体组成，G+C 含量为 52.9-53.5 mol%。基于 16S rRNA 基因序列的系统发育分析表明，MV-1(T) 菌株属于α变形菌的红螺菌科，但与 Magnetospirillum 属关系不密切。建议将菌株 MV-1(T) 命名为新属新种 Magnetovibrio blakemorei gen. nov., sp. nov.。Magnetovibrio blakemorei 的模式菌株为 MV-1(T)（=ATCC BAA-1436(T)=DSM 18854(T)）。
