LBPA, ENS de Cachan, CNRS, Cachan, France.
PLoS One. 2012;7(9):e44287. doi: 10.1371/journal.pone.0044287. Epub 2012 Sep 12.
Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non-specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated.
生物传感器表面的非特异性结合是定量分析固定化靶分子对分析物选择性保留的主要障碍。尽管已经开发出了一系列化学抗污单层来解决这个问题，但由于普遍存在的高度非特异性结合，许多大分子相互作用仍然难以分析。在本文中，我们探索了自组装单层形成的动态过程，并优化了物理和化学性质，从而在保持固定化生物分子完整性的同时，大大减少了非特异性结合。结果，固定化靶分子与分析物特异性结合的分析得到了显著促进。
