Yoshida K, Kyo E, Tsuda T, Tsujino T, Ito M, Niimoto M, Tahara E
First Department of Pathology, Hiroshima University School of Medicine,Japan.
Int J Cancer. 1990 Jan 15;45(1):131-5. doi: 10.1002/ijc.2910450124.
In order to ascertain autocrine growth factors in esophageal carcinomas, we analysed expression of mRNAs and proteins for epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha) and epidermal growth factor receptor (EGFR) in 6 esophageal carcinoma cell lines. Gene alterations were also examined. All of the esophageal carcinoma cell lines expressed mRNA for EGFR and TGF-alpha genes. Interestingly, EGF mRNA of about 5.0 kb was also detected in TE-1, TE-2, and TE-8 cells. Production of protein was also confirmed by binding assay and ELISA on 3 of the 6 cell lines. The cells had a relatively high number of EGFRs and produced TGF-alpha and EGF protein at the same time. Furthermore, anti-EGF (KEM-10) and anti-TGF-alpha (WA-3) monoclonal antibodies (MAbs) inhibited spontaneous uptake of tritiated thymidine (3H-TdR) by TE-1 cells which expressed EGF, TGF-alpha and EGFR mRNA and protein. These results strongly suggest that EGF and/or TGF-alpha produced by carcinoma cells function as autocrine growth factors for human esophageal carcinomas.
为了确定食管癌中的自分泌生长因子，我们分析了6种食管癌细胞系中表皮生长因子（EGF）、转化生长因子-α（TGF-α）和表皮生长因子受体（EGFR）的mRNA和蛋白表达情况。还检测了基因改变。所有食管癌细胞系均表达EGFR和TGF-α基因的mRNA。有趣的是，在TE-1、TE-2和TE-8细胞中还检测到约5.0 kb的EGF mRNA。通过结合试验和ELISA在6个细胞系中的3个中也证实了蛋白的产生。这些细胞具有相对较高数量的EGFR，同时产生TGF-α和EGF蛋白。此外，抗EGF（KEM-10）和抗TGF-α（WA-3）单克隆抗体（MAbs）抑制了表达EGF、TGF-α和EGFR mRNA及蛋白的TE-1细胞对氚标记胸腺嘧啶核苷（3H-TdR）的自发摄取。这些结果有力地表明，癌细胞产生的EGF和/或TGF-α作为人食管癌的自分泌生长因子发挥作用。
