表皮生长因子、转化生长因子-α及其受体基因在人胃癌中的表达；对自分泌生长的影响。
Expression of epidermal growth factor, transforming growth factor-alpha and their receptor genes in human gastric carcinomas; implication for autocrine growth.
作者信息
Yoshida K, Kyo E, Tsujino T, Sano T, Niimoto M, Tahara E
机构信息
First Department of Pathology, Hiroshima University School of Medicine.
出版信息
Jpn J Cancer Res. 1990 Jan;81(1):43-51. doi: 10.1111/j.1349-7006.1990.tb02505.x.
Abstract
The expressions of mRNA for epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha) and EGF receptor (EGFR) genes were examined in 7 human gastric carcinoma cell lines and 15 gastric carcinoma tissues and the corresponding normal mucosas. All of the gastric carcinoma cell lines expressed mRNA for EGFR and TGF-alpha genes. TMK-1 and MKN-28 cells also expressed EGF mRNA. Production of EGF, TGF-alpha and EGFR protein by gastric carcinoma cell lines was also confirmed by EGF and TGF-alpha specific monoclonal antibody binding. As for surgical specimens, EGFR and TGF-alpha mRNA were detected at high levels in all the tumor tissues. Interestingly, EGF mRNA was detected in 5 (33.3%) of the 15 gastric carcinomas but it was not detected in normal tissues. Moreover, anti-EGF and anti-TGF-alpha monoclonal antibodies inhibited the spontaneous 3H-TdR uptake by gastric carcinoma cells. These results suggest that EGF and/or TGF-alpha produced by tumor cells act as autocrine growth factors for gastric carcinomas.
摘要
在7种人胃癌细胞系、15例胃癌组织及相应的正常黏膜中检测了表皮生长因子（EGF）、转化生长因子-α（TGF-α）和EGF受体（EGFR）基因的mRNA表达。所有胃癌细胞系均表达EGFR和TGF-α基因的mRNA。TMK-1和MKN-28细胞也表达EGF mRNA。通过EGF和TGF-α特异性单克隆抗体结合也证实了胃癌细胞系产生EGF、TGF-α和EGFR蛋白。至于手术标本，在所有肿瘤组织中均检测到高水平的EGFR和TGF-α mRNA。有趣的是，在15例胃癌中有5例（33.3%）检测到EGF mRNA，但在正常组织中未检测到。此外，抗EGF和抗TGF-α单克隆抗体抑制了胃癌细胞对3H-TdR的自发摄取。这些结果表明，肿瘤细胞产生的EGF和/或TGF-α作为胃癌的自分泌生长因子发挥作用。
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