Key Laboratory of Gene Engineering of the Ministry of Education and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
J Med Food. 2012 Oct;15(10):894-900. doi: 10.1089/jmf.2012.2251. Epub 2012 Sep 17.
Naringin, a well-known flavanone glycoside of grapefruit and citrus fruits, was found to be as an effective anti-inflammatory compound in our previous lipopolysaccharide-induced acute lung injury mouse model via blockading activity of nuclear factor κB. The current study sought to explore the anti-inflammatory effects of naringin on chronic pulmonary neutrophilic inflammation in cigarette smoke (CS)-induced rats. Seventy Sprague-Dawley rats were randomly divided into seven groups to study the effects of CS with or without various concentrations of naringin or saline for 8 weeks. The results revealed that naringin supplementation at 20, 40, and 80 mg/kg significantly increased body weight of CS-induced rats as compared to that in the CS group. Moreover, naringin of 20, 40, and 80 mg/kg prevented CS-induced infiltration of neutrophils and activation of myeloperoxidase and matrix metalloproteinase-9, in parallel with suppression of the release of cytokines, such as tumor necrosis factor-α and interleukin-8 (IL-8). IL-10 in bronchoalveolar lavage fluid was significantly suppressed after CS exposure, but dose dependently elevated by naringin. The results from hematoxylin and eosin staining revealed that naringin dose dependently reduced CS-induced infiltration of inflammatory cells, thickening of the bronchial wall, and expansion of average alveolar airspace. In conclusion, our data suggest that naringin is an effective anti-inflammatory compound for attenuating chronic pulmonary neutrophilic inflammation in CS-induced rats.
柚皮苷是葡萄柚和柑橘类水果中一种众所周知的类黄酮糖苷，在我们之前的脂多糖诱导的急性肺损伤小鼠模型中被发现是一种有效的抗炎化合物，通过阻断核因子 κB 的活性。本研究旨在探讨柚皮苷对香烟烟雾（CS）诱导的大鼠慢性肺中性粒细胞炎症的抗炎作用。70 只 Sprague-Dawley 大鼠随机分为 7 组，研究 CS 与不同浓度柚皮苷或生理盐水共 8 周对大鼠的影响。结果表明，与 CS 组相比，20、40 和 80mg/kg 的柚皮苷补充剂显著增加了 CS 诱导大鼠的体重。此外，20、40 和 80mg/kg 的柚皮苷可预防 CS 诱导的中性粒细胞浸润和髓过氧化物酶和基质金属蛋白酶-9 的激活，同时抑制细胞因子如肿瘤坏死因子-α和白细胞介素-8（IL-8）的释放。CS 暴露后支气管肺泡灌洗液中 IL-10 明显受到抑制，但柚皮苷呈剂量依赖性升高。苏木精-伊红染色的结果表明，柚皮苷可剂量依赖性减少 CS 诱导的炎症细胞浸润、支气管壁增厚和平均肺泡气腔扩张。总之，我们的数据表明，柚皮苷是一种有效的抗炎化合物，可减轻 CS 诱导的大鼠慢性肺中性粒细胞炎症。
