REQUIMTE, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.
J Microbiol Methods. 2012 Dec;91(3):358-60. doi: 10.1016/j.mimet.2012.09.007. Epub 2012 Sep 15.
The discovery of a novel canine norovirus was recently reported. The VP1 capsid protein of this canine norovirus was expressed in Saccharomyces cerevisiae and purified with a yield of 1.6-1.8 mg/L. The yeast-expressed VP1 protein self-assembled into virus-like particles and exhibited antigenicity, proving to be suitable for serological assays.
最近有报道称发现了一种新型犬诺如病毒。该犬诺如病毒的 VP1 衣壳蛋白在酿酒酵母中表达，并以 1.6-1.8mg/L 的产率进行纯化。酵母表达的 VP1 蛋白自行组装成病毒样颗粒，并表现出抗原性，证明适用于血清学检测。
