Department of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh; Department of Biochemistry, Pt. Jawahar Lal Nehru Government Medical College, Chamba, Himachal Pradesh, India.
Department of Gastroenterology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Indian J Pharmacol. 2022 Jul-Aug;54(4):270-277. doi: 10.4103/ijp.ijp_90_22.
The intestinal permeability (IP) of sugars and their derivatives has been widely used to assess mucosal damage in gastrointestinal diseases. Ulcerative colitis (UC) is a recurring and relapsing disease that causes inflammation of the gut. IP of sugars can be evaluated and correlated with the flare of UC.
A prospective study was conducted on 91 patients with active UC at the tertiary care center in North India. Mayo grading system assessed disease activity, and IP was assessed by measuring sucrose, lactulose, mannitol, and sucralose in urine samples from UC patients. A high-performance liquid chromatography (HPLC) method to detect all of these sugars simultaneously using a refractive index detector was developed and further validated in patients with UC.
The analytical recovery rate of the tested sugars ranged from 95% to 146% in the urine matrix. The limit of detection and limit of quantification were 78.838 mg/L and 262.79 mg/L for sucrose, 84.994 mg/L and 283.31 mg/L for lactulose, 74.789 mg/L and 249.30 mg/L for mannitol, and 50.908 mg/L and 169.69 mg/L for sucralose.
The standardized HPLC method is sensitive and suitable for the simultaneous detection and determination of different sugar moieties in the urine sample. Patients with UC can be evaluated indirectly for the flare by estimating the recovery rate of sugars through gut permeability. The procedure is noninvasive and thus improves the quality of life of chronically ill patients.
糖及其衍生物的肠道通透性（IP）已广泛用于评估胃肠道疾病中的粘膜损伤。溃疡性结肠炎（UC）是一种反复发作和复发的疾病，会导致肠道炎症。可以评估糖的 IP 并将其与 UC 的发作相关联。
在印度北部的三级护理中心对 91 例活动性 UC 患者进行了前瞻性研究。Mayo 分级系统评估疾病活动度，通过测量 UC 患者尿液样本中的蔗糖、乳果糖、甘露醇和三氯蔗糖来评估 IP。开发了一种同时使用折射指数检测器检测所有这些糖的高效液相色谱（HPLC）方法，并在 UC 患者中进一步验证。
在尿液基质中，测试糖的分析回收率范围为 95%至 146%。蔗糖的检测限和定量限分别为 78.838 mg/L 和 262.79 mg/L，乳果糖为 84.994 mg/L 和 283.31 mg/L，甘露醇为 74.789 mg/L 和 249.30 mg/L，三氯蔗糖为 50.908 mg/L 和 169.69 mg/L。
标准化 HPLC 方法灵敏，适用于尿液样本中不同糖部分的同时检测和定量。可以通过估计糖的肠道通透性回收率来间接评估 UC 患者的发作情况。该程序是非侵入性的，从而提高了慢性病患者的生活质量。
