Robert Koch Institute, Centre for Biological Security, Berlin, Germany, European Union.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2012 Oct;114(4):480-6. doi: 10.1016/j.oooo.2012.06.006.
Recently, specific in vitro bisphosphonate concentrations have been established for reaching a toxic threshold that could result in the induction of bisphosphonate-related osteonecrosis of the jaw (BRONJ), but these data have not been validated in vivo. The purpose of this study was to quantify the concentration of bisphosphonates (BPs) in the diseased jaw bone of patients experiencing BRONJ.
We hypothesized that if the average natural nitrogen content of mammalian bone is known, the excess of nitrogen in the jaw bone of BRONJ patients is likely to reflect the concentration of amino-BP. To test our hypothesis, jaw bone specimens from patients with BRONJ were acquired after sequestrectomy and analyzed by energy-dispersive X-ray spectroscopy (EDS).
The EDS analysis of the bone demonstrated a highly linear correlation between increasing concentrations of BP and the increasing percentage of nitrogen measured at the bone surfaces (R(2) = .9851, P = .0149).
SEM/EDS can be a valuable tool for assessing BP concentration in jaw bone and provides important insight into BP pharmacokinetics and BRONJ.
最近，已经确定了特定的体外双膦酸盐浓度，以达到可能导致双膦酸盐相关性下颌骨坏死（BRONJ）的毒性阈值，但这些数据尚未在体内得到验证。本研究的目的是定量测定发生 BRONJ 的患者病颌骨中的双膦酸盐（BPs）浓度。
我们假设，如果已知哺乳动物骨骼的平均天然氮含量，BRONJ 患者颌骨中的过量氮很可能反映了氨基-BP 的浓度。为了验证我们的假设，在施行清创术后从 BRONJ 患者中获得颌骨标本，并通过能谱 X 射线光谱仪（EDS）进行分析。
对骨骼的 EDS 分析表明，BP 浓度的增加与在骨表面测量的氮百分比的增加之间呈高度线性相关（R(2) =.9851，P =.0149）。
SEM/EDS 可以成为评估颌骨中 BP 浓度的有价值工具，并为 BP 药代动力学和 BRONJ 提供重要的见解。
