考林蛋白水平调节依托泊苷处理的 U2OS 细胞中的细胞周期进程和 γH2AX 水平。
Coilin levels modulate cell cycle progression and γH2AX levels in etoposide treated U2OS cells.
机构信息
Department of Biochemistry, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA.
出版信息
FEBS Lett. 2012 Sep 21;586(19):3404-9. doi: 10.1016/j.febslet.2012.07.054. Epub 2012 Aug 7.
Abstract
Coilin is considered the Cajal body (CB) marker protein. In this report, we investigated the role of coilin in the DNA damage response and found that coilin reduction correlated with significantly increased levels of soluble γH2AX in etoposide treated U2OS cells. Additionally, coilin levels influenced the proliferation rate and cell cycle distribution of cells exposed to etoposide. Moreover, coilin overexpression inhibited nucleolar localization of endogenous coilin in etoposide treated U2OS cells. Collectively, these data provide additional evidence for coilin and CBs in the DNA damage response.
摘要
科尔林被认为是 Cajal 体（CB）标志物蛋白。在本报告中，我们研究了科尔林在 DNA 损伤反应中的作用，发现依托泊苷处理的 U2OS 细胞中科尔林减少与可溶性 γH2AX 水平显著增加相关。此外，科尔林水平影响暴露于依托泊苷的细胞的增殖率和细胞周期分布。此外，科尔林过表达抑制了依托泊苷处理的 U2OS 细胞中内源性科尔林的核仁定位。总之，这些数据为科尔林和 CB 在 DNA 损伤反应中的作用提供了额外的证据。
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