Machyna Martin, Neugebauer Karla M, Staněk David
a Department of Molecular Biophysics & Biochemistry ; Yale University ; New Haven , CT USA.
RNA Biol. 2015;12(6):590-6. doi: 10.1080/15476286.2015.1034923.
Initially identified as a marker of coiled bodies (now Cajal bodies or CBs), the protein coilin was discovered a quarter of century ago. Coilin is now known to scaffold the CB, but its structure and function are poorly understood. Nearly devoid of predicted structural motifs, coilin has numerous reported molecular interactions that must underlie its role in the formation and function of CBs. In this review, we summarize what we have learned in the past 25 years about coilin's structure, post-transcriptional modifications, and interactions with RNA and proteins. We show that genes with homology to human coilin are found in primitive metazoans and comment on differences among model organisms. Coilin's function in Cajal body formation and RNP metabolism will be discussed in the light of these developments.
蛋白质卷曲螺旋蛋白最初被鉴定为螺旋体（现称卡哈尔体或CBs）的标志物，于四分之一个世纪前被发现。目前已知卷曲螺旋蛋白构成了卡哈尔体的支架，但其结构和功能仍知之甚少。卷曲螺旋蛋白几乎没有预测的结构基序，却有大量已报道的分子相互作用，这些相互作用必定是其在卡哈尔体形成和功能中发挥作用的基础。在这篇综述中，我们总结了过去25年里我们对卷曲螺旋蛋白的结构、转录后修饰以及与RNA和蛋白质相互作用的了解。我们表明，与人类卷曲螺旋蛋白具有同源性的基因存在于原始后生动物中，并对模式生物之间的差异进行了评论。将根据这些进展讨论卷曲螺旋蛋白在卡哈尔体形成和核糖核蛋白代谢中的功能。
