School of Life Sciences, Arizona State University, Main Campus, Tempe, AZ 85287-4501, USA.
Antonie Van Leeuwenhoek. 2013 Feb;103(2):321-30. doi: 10.1007/s10482-012-9812-1. Epub 2012 Sep 18.
Strain OR362-8(T) was isolated from a biological soil crust sample collected from the southwestern arid lands of the United States of America, using BG11-PGY medium. Cells of OR362-8(T) were found to be rod shaped; occur singly, as pairs and in groups; non-motile; positive for catalase, oxidase, phosphatase and gelatinase; hydrolyze starch; contain iso-C(15:0), anteiso-C(15:0), iso-C(15:1)G, C(16:1ω5c) and summed feature 3 (C(16:1(ω7c))/iso-C(15:0) 2OH as defined by the MIDI system) as the major fatty acids; and MK-7 as the sole respiratory quinone. A BLAST sequence similarity search using 16S rRNA gene sequence of OR362-8(T) identified Hymenobacter as the nearest genus with a similarity of 90.4-96.9 %. The phylogenetic analyses based on the phenetic methods UPGMA, NJ, ME and DNA parsimony resulted in the clustering of OR362-8(T) with Clade 1 Hymenobacter species represented by Hymenobacter glaciei, Hymenobacter antarcticus, Hymenobacter flocculans, Hymenobacter metalli and Hymenobacter soli with the closest being the Hymenobacter glaciei (96.9 % 16S rRNA gene sequence similarity). Besides the strong phylogentic affiliation, OR362-8(T) also exhibited significant phenotypic and chemotaxonomic differences with the members of Clade 1 Hymenobacter spp. More importantly, the DNA G+C content (mol%) of OR362-8(T) is very high (70 %) compared to the nearest species identified by phylogenetic analysis. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, OR362-8(T) was assigned to a novel species for which we propose here the name Hymenobacter arizonensis sp. nov., with OR362-8(T) (=ATCC BAA 1266(T) = DSM 17860(T) = JCM 13504(T)) as the type strain.
OR362-8(T) 菌株是从美国西南部干旱地区的生物土壤结皮样本中分离出来的，使用 BG11-PGY 培养基进行培养。OR362-8(T) 细胞呈杆状；单独存在，成对存在，成群存在；不运动；过氧化氢酶、氧化酶、磷酸酶和明胶酶阳性；水解淀粉；含有异-C(15:0)、anteiso-C(15:0)、iso-C(15:1)G、C(16:1ω5c)和总和特征 3 (C(16:1(ω7c))/iso-C(15:0) 2OH，按 MIDI 系统定义)作为主要脂肪酸；以及 MK-7 作为唯一的呼吸醌。使用 OR362-8(T) 的 16S rRNA 基因序列进行 BLAST 序列相似性搜索，发现 Hymenobacter 是最接近的属，相似度为 90.4-96.9%。基于 phenetic 方法 UPGMA、NJ、ME 和 DNA 简约性的系统发育分析导致 OR362-8(T) 与 Hymenobacter glaciei、Hymenobacter antarcticus、Hymenobacter flocculans、Hymenobacter metalli 和 Hymenobacter soli 代表的 Clade 1 Hymenobacter 物种聚类，最接近的是 Hymenobacter glaciei(96.9% 16S rRNA 基因序列相似性)。除了强烈的系统发育关系外，OR362-8(T) 还表现出与 Clade 1 Hymenobacter spp. 的显著表型和化学分类学差异。更重要的是，OR362-8(T) 的 DNA G+C 含量(mol%)非常高(70%)，与系统发育分析鉴定的最接近的物种相比。基于系统发育、表型和化学分类学特征，OR362-8(T) 被分配到一个新的物种，我们在这里提出了 Hymenobacter arizonensis sp. nov.的名称，其中 OR362-8(T)=ATCC BAA 1266(T)=DSM 17860(T)=JCM 13504(T) 作为模式菌株。
