Srinivasan Sathiyaraj, Joo Eun Sun, Lee Jae-Jin, Kim Myung Kyum
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Korea.
Antonie Van Leeuwenhoek. 2015 Jun;107(6):1411-9. doi: 10.1007/s10482-015-0436-0. Epub 2015 Mar 31.
A red-pink coloured, Gram-negative, rod-shaped bacterium designated as strain DG31A(T) was isolated from soil collected in Seoul, South Korea. The isolate was found to grow optimally at 25 °C on R2A agar. The highest degrees of 16S rRNA gene sequence similarities of the strain were found with Hymenobacter arizonensis JCM 13504(T) (98.0 %), Hymenobacter glaciei VUG-A130(T) (96.1 %), Hymenobacter soli PB17(T) (95.2 %), Hymenobacter antarcticus VUG-A42aa(T) (94.7 %) and Hymenobacter chitinivorans Txc1(T) (92.8 %). The DNA G+C content of the novel strain, DG31A(T), was determined to be 60.8 mol%. Chemotaxonomic data revealed that the major fatty acids were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c; 26.7 %), C16:1 ω5c (18.9 %) and anteiso-C15:0 (12.9 %); the major polar lipid was identified as phosphatidylethanolamine; the polyamine pattern was found to contain sym-homospermidine; and the major quinone was identified as MK-7. The DNA-DNA relatedness of strain DG31A(T) with respect to H. arizonensis JCM 13504(T) was 19.5 ± 2.9 % (reciprocal, 19.3 ± 0.6 %). Based on these data, strain DG31A(T) should be classified within the genus Hymenobacter as a novel species for which the name Hymenobacter humi sp. nov. is proposed, with the type strain DG31A(T) (=KCTC 32523(T) = JCM 19635(T)).
从韩国首尔采集的土壤中分离出一株呈红粉色、革兰氏阴性、杆状的细菌，命名为菌株DG31A(T)。该分离株在R2A琼脂培养基上于25°C时生长最佳。该菌株16S rRNA基因序列与亚利桑那膜杆菌JCM 13504(T)（98.0%）、冰川膜杆菌VUG-A130(T)（96.1%）、土壤膜杆菌PB17(T)（95.2%）、南极膜杆菌VUG-A42aa(T)（94.7%）和几丁质食膜杆菌Txc1(T)（92.8%）的相似性最高。新菌株DG31A(T)的DNA G+C含量测定为60.8 mol%。化学分类数据显示，主要脂肪酸为总和特征3（C16:1 ω7c和/或C16:1 ω6c；26.7%）、C16:1 ω5c（18.9%）和anteiso-C15:0（12.9%）；主要极性脂被鉴定为磷脂酰乙醇胺；多胺模式含有共生高亚精胺；主要醌被鉴定为MK-7。菌株DG31A(T)与亚利桑那膜杆菌JCM 13504(T)的DNA-DNA相关性为19.5±2.9%（相互，19.3±0.6%）。基于这些数据，菌株DG31A(T)应归为膜杆菌属中的一个新物种，提议将其命名为土壤膜杆菌（Hymenobacter humi sp. nov.），模式菌株为DG31A(T)（=KCTC 32523(T)=JCM 19635(T)）。
