Department of Otolaryngology-Head and Neck Surgery, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Head Neck. 2013 Aug;35(8):1119-23. doi: 10.1002/hed.23105. Epub 2012 Sep 18.
We present our experience with the use of an immunocompetent medium-sized animal model of tongue cancer that may be suitable for imaging and surgical studies.
A New Zealand white rabbit model of tongue cancer was created by injecting a VX tumor cell suspension grown in culture into the tongue of our model. The tumor was examined 7 days following implantation by physical examination, photography, and (18) fluoro 2-deoxyglucose-positron emission tomography (FDG-PET). At 12 days postimplantation, the model was again studied as described above prior to euthanization, and then tongue excision and bilateral neck dissections were performed. All tissue was examined by histology.
We confirmed a successful orthotopic tongue cancer model that resulted in cervical nodal metastases.
This model may be a useful model of orthotopic head and neck cancer for future surgical or imaging research.
我们介绍了一种可用于成像和外科研究的免疫功能正常的中型舌癌动物模型的使用经验。
通过将在培养中生长的 VX 肿瘤细胞混悬液注入新西兰白兔的舌中，建立了舌癌动物模型。在植入后 7 天，通过体格检查、摄影和（18）氟-2-脱氧葡萄糖正电子发射断层扫描（FDG-PET）对肿瘤进行检查。在植入后 12 天，对模型进行了如上所述的再次研究，然后进行安乐死，并进行舌切除和双侧颈部淋巴结清扫。所有组织均通过组织学检查。
我们证实了一种成功的原位舌癌模型，导致了颈部淋巴结转移。
该模型可能是未来头颈部癌症外科或成像研究的有用模型。
