Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, 34118 Istanbul, Turkey.
Evid Based Complement Alternat Med. 2012;2012:958740. doi: 10.1155/2012/958740. Epub 2012 Aug 16.
Viscum album L. is a semiparasitic plant grown on trees and widely used for the treatment of many diseases in traditional and complementary therapy. It is well known that some activities of Viscum album extracts are varied depending on the host trees, such as antioxidant, apoptosis-inducing, anticancer activities of the plant. The aim of the present study is to examine the comparative effects of methanolic extracts of V. album grown on three different host trees (locust tree, lime tree, and hedge maple tree) on H(2)O(2)-induced DNA damage in HeLa cells. Oxidative damage in mitochondrial DNA and two nuclear regions was assessed by QPCR assay. The cells were pretreated with methanolic extracts (10 μg/mL) for 48 h, followed by the treatment with 750 μM H(2)O(2) for 1 hour. DNA damage was significantly induced by H(2)O(2) while it was inhibited by V. album extracts. All extracts completely protected against nuclear DNA damage. While the extract from lime tree or white locust tree entirely inhibited mitochondrial DNA damage, that from hedge maple tree inhibited by only 50%. These results suggest that methanolic extracts of V. album can prevent oxidative DNA damage, and the activity is dependent on the host tree.
槲寄生是一种半寄生植物，生长在树上，广泛用于传统和补充疗法治疗许多疾病。众所周知，槲寄生提取物的一些活性因宿主树而异，如植物的抗氧化、诱导细胞凋亡和抗癌活性。本研究旨在研究生长在三种不同宿主树上（槐树、椴树和山毛榉树）的槲寄生甲醇提取物对 H 2 O 2 诱导的 HeLa 细胞 DNA 损伤的比较影响。通过 QPCR 检测线粒体 DNA 和两个核区的氧化损伤。细胞用甲醇提取物（10μg/ml）预处理 48 小时，然后用 750μM H 2 O 2 处理 1 小时。H 2 O 2 显著诱导 DNA 损伤，而槲寄生提取物则抑制 DNA 损伤。所有提取物均能完全防止核 DNA 损伤。虽然来自椴树或白槐树的提取物完全抑制了线粒体 DNA 损伤，但来自山毛榉树的提取物仅抑制了 50%。这些结果表明，槲寄生甲醇提取物可以预防氧化 DNA 损伤，其活性取决于宿主树。
