Department of Pharmacy, Pharmacology and Toxicology, University of Veterinary Medicine, Kosice, Slovak Republic.
Phytother Res. 2010 Mar;24(3):365-8. doi: 10.1002/ptr.2947.
Mistletoe preparations are frequently used by cancer patients because of their ability to stimulate the immunity and to improve the quality of life. Moreover mistletoe and its active substances (especially lectins) possess cytotoxic effect on various cancer cell lines. However, only little is known about its interaction with anticancer drugs. Therefore the cytotoxic and apoptosis-inducing effects of aqueous mistletoe extract (VA) and its interaction with doxorubicin (DOXO) were investigated in Jurkat cells. The results show that VA extract as well as DOXO exert cytotoxic effects on Jurkat cells in a dose-dependent manner. Cytotoxicity of DOXO was much stronger (LC(50) = 11.68 ng/mL) than that of VA extract (LC(50) = 35.67 microg/mL). Their combination led to synergism only at those concentrations that were highly cytotoxic alone. Both substances (alone and in combination) induced DNA fragmentation in Jurkat cells. In conclusion, an aqueous extract prepared from mistletoe tops exerted cytotoxic and apoptosis-inducing effects on Jurkat cells alone as well as in combination with DOXO.
槲寄生制剂常被癌症患者使用，因为它们能够刺激免疫力并提高生活质量。此外，槲寄生及其活性物质（特别是凝集素）对多种癌细胞系具有细胞毒性作用。然而，人们对其与抗癌药物的相互作用知之甚少。因此，本研究旨在研究水提槲寄生提取物（VA）及其与阿霉素（DOXO）的相互作用对 Jurkat 细胞的细胞毒性和诱导细胞凋亡的作用。结果表明，VA 提取物和 DOXO 均以剂量依赖的方式对 Jurkat 细胞产生细胞毒性作用。DOXO 的细胞毒性要强得多（LC50=11.68ng/mL），而 VA 提取物的细胞毒性较弱（LC50=35.67μg/mL）。只有在高浓度下，两者的联合才具有协同作用。这两种物质（单独和联合使用）均能诱导 Jurkat 细胞发生 DNA 片段化。总之，槲寄生顶部的水提物单独以及与 DOXO 联合使用均可对 Jurkat 细胞产生细胞毒性和诱导细胞凋亡的作用。
