Giunta C, De Bortoli M, Brigidi R
Boll Soc Ital Biol Sper. 1979 Sep 15;55(17):1659-65.
We attempted separations by affinity chromatography of the Na+/K+-dependent adenosinetriphosphatase, using Sepharose 6B covalently coupled with ouabain, either on microsomal fractions or on preparations purified by discontinuous sucrose density gradient ultracentrifugation. The material, specifically eluted with ouabain, was tested to evaluate the recovery of ATPase activity and ouabain binding capacity. The results have shown the efficiency of this technique for (Na+/K+)-APTase isolation.
我们尝试通过亲和色谱法分离钠钾依赖型三磷酸腺苷酶，使用与哇巴因共价偶联的琼脂糖6B，分别作用于微粒体部分或通过不连续蔗糖密度梯度超速离心纯化的制剂。用哇巴因特异性洗脱的物质，经过测试以评估三磷酸腺苷酶活性和哇巴因结合能力的回收率。结果表明了该技术用于分离（钠/钾）-三磷酸腺苷酶的有效性。
