Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, UP, India.
Adv Genet. 2012;79:87-121. doi: 10.1016/B978-0-12-394395-8.00003-7.
Next-generation sequencing strategies have opened new vistas for molecular plant breeding. The sequence information obtained by the advent of next-generation sequencing provides a valuable tool not only for improving domesticated crops but also for investigating the natural evolution of crops. Such information provides an enormous potential for sustainable agriculture. In this review, we discuss how such sequencing approaches have transformed exome sequencing into a practical utility that has enormous potential for crop improvement in agriculture. Furthermore, we also describe the future of crop improvement beyond the exome sequencing strategies.
下一代测序策略为分子植物育种开辟了新的前景。随着下一代测序的出现，所获得的序列信息不仅为改良驯化作物提供了宝贵的工具，也为研究作物的自然进化提供了线索。这些信息为可持续农业提供了巨大的潜力。在这篇综述中，我们讨论了这些测序方法如何将外显子组测序转化为一种实用工具，在外显子组测序策略之外，这种方法在农业改良作物方面具有巨大的潜力。
