Ministry of Education Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China.
Planta. 2013 Jan;237(1):137-44. doi: 10.1007/s00425-012-1763-2. Epub 2012 Sep 19.
Elevated carbon dioxide (CO₂) has been shown to enhance the growth and development of plants, especially of roots. Amongst them, lateral roots play an important role in nutrient uptake, and thus alleviate the nutrient limitation to plant growth under elevated CO₂. This paper examined the mechanism underlying CO₂ elevation-induced lateral root formation in tomato. The endogenous nitric oxide (NO) in roots was detected by the specific probe 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate (DAF-FM DA). We suggest that CO₂ elevation-induced NO accumulation was important for lateral root formation. Elevated CO₂ significantly increased the activity of nitric oxide synthase in roots, but not nitrate reductase activity. Moreover, the pharmacological evidence showed that nitric oxide synthase rather than nitrate reductase was responsible for CO₂ elevation-induced NO accumulation. Elevated CO₂ enhanced the activity of nitric oxide synthase and promoted production of NO, which was involved in lateral root formation in tomato under elevated CO₂.
二氧化碳（CO₂）升高已被证明能促进植物，尤其是根部的生长和发育。其中，侧根在养分吸收中起着重要作用，从而缓解了 CO₂升高对植物生长的养分限制。本文研究了 CO₂升高诱导番茄侧根形成的机制。用特异性探针 4-氨基-5-甲基氨基-2'，7'-二氟荧光素二乙酸酯（DAF-FM DA）检测根部内源性一氧化氮（NO）。我们认为 CO₂升高诱导的 NO 积累对侧根形成很重要。CO₂升高显著增加了根部一氧化氮合酶的活性，但硝酸还原酶的活性没有增加。此外，药理学证据表明，NO 合酶而不是硝酸还原酶负责 CO₂升高诱导的 NO 积累。CO₂升高增强了一氧化氮合酶的活性，促进了 NO 的产生，这参与了 CO₂升高下番茄侧根的形成。
