Department of Animal Sciences and Technology, Shandong Agricultural University, Shandong, People's Republic of China.
Poult Sci. 2012 Oct;91(10):2487-95. doi: 10.3382/ps.2012-02437.
The objectives of the present study were to investigate the toxicity of feed-borne Fusarium mycotoxins on organ weight, serum biochemistry, and immunological parameters of broiler chickens and to evaluate the efficacy of yeast cell wall adsorbent in preventing mycotoxin-induced adverse effects. In total, 300 one-day-old vaccinated (Marek's disease and infectious bronchitis) Arbor Acres broiler chickens (mixed sex) were randomly divided into 3 treatments (5 repetitions per treatment) and fed basal diet and naturally contaminated diets with or without yeast cell wall adsorbent. Treatments were control, naturally contaminated diet (NCD; aflatoxin, 102.08 mg/kg; zearalenone, 281.92 mg/kg; fumonisin, 5,874.38 mg/kg; deoxynivalenol, 2,038.96 mg/kg), and NCD + 2 g/kg of yeast cell wall adsorbent (NCDD). The test included 2 phases: d 0-21 and d 22-42. At 42 d, broilers fed contaminated diets without yeast cell wall adsorbent had higher (P < 0.05) serum albumin and higher relative weight of liver, bursa of Fabricius, and thymus, and greater splenic mRNA expression of IL-1β and IL-6 at 42 d compared with the control, but lower (P < 0.05) serum globulin at 42 d, IgA at 21 d, relative weight of spleen at 21 d, antibody titers of Newcastle disease at both 28 d and 42 d, and splenic mRNA expression of IFN-γ at 42 d were observed in the NCD treatment compared with control. Dietary addition of yeast cell wall adsorbent in the NCD treatment showed a positive protection effect on the relative weight of the liver and spleen at 21 d, relative weight of the bursa of Fabricius and thymus at 42 d, antibody titers of Newcastle disease at both 28 d and 42 d, and splenic mRNA expression of IL-1β, IL-6, and IFN-γ at 42 d. It is suggested that feeding a naturally contaminated diet for 42 d might result in a deleterious effect in broiler chickens, and addition of 2 g/kg of yeast cell wall enterosorbent can partly neutralize the detrimental effects of the naturally contaminated feed.
本研究的目的是研究饲料源性镰刀菌霉菌毒素对肉鸡器官重量、血清生化和免疫参数的毒性，并评估酵母细胞壁吸附剂预防霉菌毒素引起的不良影响的功效。总共将 300 只 1 日龄接种（马立克氏病和传染性支气管炎）阿伯丁肉鸡（雌雄混合）随机分为 3 个处理组（每个处理 5 个重复），并饲喂基础日粮和受自然污染的日粮，或添加或不添加酵母细胞壁吸附剂。处理组分别为对照组、自然污染日粮组（黄曲霉毒素 102.08mg/kg、玉米赤霉烯酮 281.92mg/kg、伏马菌素 5874.38mg/kg、脱氧雪腐镰刀菌烯醇 2038.96mg/kg）和自然污染日粮+2g/kg 酵母细胞壁吸附剂组（NCDD）。试验包括 2 个阶段：第 0-21 天和第 22-42 天。在第 42 天，与对照组相比，饲喂未添加酵母细胞壁吸附剂的受污染日粮的肉鸡血清白蛋白更高（P<0.05），肝脏、法氏囊和胸腺的相对重量更高，第 42 天脾脏白细胞介素-1β和白细胞介素-6 的 mRNA 表达更高，但血清球蛋白更低（P<0.05），第 42 天 IgA、第 21 天脾脏相对重量、第 28 天和第 42 天新城疫抗体效价、第 42 天脾脏 IFN-γ 的 mRNA 表达均较低。在 NCD 处理中添加酵母细胞壁吸附剂对第 21 天肝脏和脾脏的相对重量、第 42 天法氏囊和胸腺的相对重量、第 28 天和第 42 天新城疫抗体效价以及第 42 天脾脏白细胞介素-1β、白细胞介素-6 和 IFN-γ 的 mRNA 表达均有积极的保护作用。提示连续饲喂 42 天自然污染日粮可能会对肉鸡造成有害影响，添加 2g/kg 酵母细胞壁肠吸附剂可部分中和自然污染饲料的有害作用。
