用于评估受精能力的精子穿透试验。
The sperm penetration assay for the assessment of fertilization capacity.
作者信息
Hwang Kathleen, Lamb Dolores J
机构信息
Department of Surgery (Urology), The Alpert Medical School of Brown University, Providence, RI, USA.
出版信息
Methods Mol Biol. 2013;927:103-11. doi: 10.1007/978-1-62703-038-0_10.
Abstract
The sperm penetration assay, or zona-free hamster oocyte penetration assay, is utilized to measure the ability of sperm to undergo capacitation, fuse with the egg membrane, and decondense the sperm head within the cytoplasm of the oocyte, resulting in the formation of the male pronucleus. The test is scored by calculation of the percentage of ova that are penetrated (the original assay developed) or the average number of sperm penetrations per ovum (the sperm capacitation index of the optimized assay). The sperm penetration assay identifies those couples that will have a high likelihood of success with in vitro fertilization.
摘要
精子穿透试验，即无透明带仓鼠卵母细胞穿透试验，用于评估精子获能、与卵膜融合以及在卵母细胞胞质内使精子头部解聚以形成雄原核的能力。该试验通过计算被穿透卵子的百分比（最初开发的试验）或每个卵子的平均精子穿透数（优化试验的精子获能指数）来评分。精子穿透试验可识别那些体外受精成功可能性高的夫妇。
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