用于评估受精能力的精子穿透试验。
The sperm penetration assay for the assessment of fertilization capacity.
作者信息
Hwang Kathleen, Lamb Dolores J
机构信息
Department of Surgery (Urology), The Alpert Medical School of Brown University, Providence, RI, USA.
出版信息
Methods Mol Biol. 2013;927:103-11. doi: 10.1007/978-1-62703-038-0_10.
Abstract
The sperm penetration assay, or zona-free hamster oocyte penetration assay, is utilized to measure the ability of sperm to undergo capacitation, fuse with the egg membrane, and decondense the sperm head within the cytoplasm of the oocyte, resulting in the formation of the male pronucleus. The test is scored by calculation of the percentage of ova that are penetrated (the original assay developed) or the average number of sperm penetrations per ovum (the sperm capacitation index of the optimized assay). The sperm penetration assay identifies those couples that will have a high likelihood of success with in vitro fertilization.
摘要
精子穿透试验，即无透明带仓鼠卵母细胞穿透试验，用于评估精子获能、与卵膜融合以及在卵母细胞胞质内使精子头部解聚以形成雄原核的能力。该试验通过计算被穿透卵子的百分比（最初开发的试验）或每个卵子的平均精子穿透数（优化试验的精子获能指数）来评分。精子穿透试验可识别那些体外受精成功可能性高的夫妇。
相似文献
1
The sperm penetration assay for the assessment of fertilization capacity.
Methods Mol Biol. 2013;927:103-11. doi: 10.1007/978-1-62703-038-0_10.
2
The relationship between critical evaluation of sperm morphology and the TYB-optimized zona free hamster oocyte sperm penetration assay.
Int J Androl. 1999 Oct;22(5):329-35. doi: 10.1046/j.1365-2605.1999.00188.x.
3
The effect of porcine follicular fluid on the interaction of boar spermatozoa with zona-free hamster ova.
Can J Vet Res. 1991 Jul;55(3):212-9.
4
Ejaculate-hormonal traits in the leopard cat (Felis bengalensis) and sperm function as measured by in vitro penetration of zona-free hamster ova and zona-intact domestic cat oocytes.
Mol Reprod Dev. 1990 Jun;26(2):163-74. doi: 10.1002/mrd.1080260211.
5
Sperm-zona binding and sperm-oocyte penetration analysed by DNA fluorescence: a test for gamete quality after in-vitro fertilization.
Hum Reprod. 1995 Dec;10(12):3218-25. doi: 10.1093/oxfordjournals.humrep.a135892.
6
Ability of Catalonian donkey sperm to penetrate zona pellucida-free bovine oocytes matured in vitro.
Anim Reprod Sci. 2010 Apr;118(2-4):354-61. doi: 10.1016/j.anireprosci.2009.08.005. Epub 2009 Aug 21.
7
Kinetics of sperm penetration and fertilization in vitro in hamster follicular and oviductal ova.
J Exp Zool. 1996 Apr 15;274(6):373-83. doi: 10.1002/(SICI)1097-010X(19960415)274:6<373::AID-JEZ6>3.0.CO;2-L.
8
Effect of liquid storage and cryopreservation of boar spermatozoa on acrosomal integrity and the penetration of zona-free hamster ova in vitro.
Gamete Res. 1987 Mar;16(3):193-204. doi: 10.1002/mrd.1120160302.
9
Evaluation of sperm subpopulation structure in relation to in vitro sperm-oocyte interaction of frozen-thawed semen from Holstein bulls.
Theriogenology. 2014 May;81(8):1067-72. doi: 10.1016/j.theriogenology.2014.01.033. Epub 2014 Jan 31.
10
Conditions influencing human sperm capacitation and penetration of zona-free hamster ova.
Fertil Steril. 1984 Apr;41(4):603-8. doi: 10.1016/s0015-0282(16)47785-x.
引用本文的文献
1
Using Cholesterol-Loaded Cyclodextrin to Improve Cryo-Survivability and Reduce Capacitation-Like Changes in Gender-Ablated Jersey Semen.
Animals (Basel). 2025 Jul 11;15(14):2038. doi: 10.3390/ani15142038.
2
The hamster egg penetration test may decrease intracytoplasmic sperm injection utilization while maintaining high conventional fertilization rates.
Asian J Androl. 2021 Jan-Feb;23(1):11-15. doi: 10.4103/aja.aja_18_20.
3
Contributions of Ryuzo Yanagimachi to the field of reproductive biology.
Biol Reprod. 2019 Jan 1;100(1):1-7. doi: 10.1093/biolre/ioy191.
4
Host genes regulate transcription of sperm-introduced hepatitis B virus genes in embryo.
Reprod Toxicol. 2017 Oct;73:158-166. doi: 10.1016/j.reprotox.2017.08.009. Epub 2017 Aug 16.
5
Cross-species fertilization: the hamster egg receptor, Juno, binds the human sperm ligand, Izumo1.
Philos Trans R Soc Lond B Biol Sci. 2015 Feb 5;370(1661):20140101. doi: 10.1098/rstb.2014.0101.
本文引用的文献
1
Male partner screening before in vitro fertilization: preselecting patients who require intracytoplasmic sperm injection with the sperm penetration assay.
Fertil Steril. 2001 Dec;76(6):1113-8. doi: 10.1016/s0015-0282(01)02890-4.
2
Human sperm microinjection into hamster oocytes: a new tool for training and evaluation of the technical proficiency of intracytoplasmic sperm injection.
Fertil Steril. 2000 Feb;73(2):395-401. doi: 10.1016/s0015-0282(99)00500-2.
3
A quality control system for the optimized sperm penetration assay.
Fertil Steril. 1995 Oct;64(4):832-7. doi: 10.1016/s0015-0282(16)57862-5.
4
The microsperm penetration assay: development of a sperm penetration assay suitable for oligospermic males.
Fertil Steril. 1991 Sep;56(3):528-34.
5
Incidence and main causes of infertility in a resident population (1,850,000) of three French regions (1988-1989).
Hum Reprod. 1991 Jul;6(6):811-6. doi: 10.1093/oxfordjournals.humrep.a137433.
6
Prediction of in vitro fertilization outcome by sperm penetration assay with TEST-yolk buffer preincubation.
Fertil Steril. 1992 Sep;58(3):556-62. doi: 10.1016/s0015-0282(16)55263-7.
7
The use of zona-free animal ova as a test-system for the assessment of the fertilizing capacity of human spermatozoa.
Biol Reprod. 1976 Nov;15(4):471-6. doi: 10.1095/biolreprod15.4.471.
Zotero 插件