Zeng Linchai, Gao Jie, Zhang Rui
The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
Zhongguo Zhong Yao Za Zhi. 2012 Jun;37(11):1651-4.
To investigate the effect and the mechanism of cyanidin-3-glucoside (C3G) in the growth inhibition of ovarian cancer in vitro and in vivo.
After human ovarian cancer cell line HO-8910PM was treated with C3G, cell growth was determined by the Cell Counting Kit-8 (CCK-8) assay and apoptosis was evaluated by flow cytometry analysis stained with Annexin V-FITC/PI. The protein expression in HO-8910PM cells was analyzed by Western blot assay. HO-8910PM cells were injected subcutaneously into nude mice to establish xenograft model. After 3 weeks of implantation, mice were randomized into 2 groups (n = 8): control group, feed with 0.2 mL double distilled water; C3G group, feed with C3G at a dose of 5 mg x kg(-1). All treatment lasted for two weeks, thrice per week. Eight weeks after implantation, tumor weight and inhibition rate were evaluated respectively after the mice were sacrificed. Immunohistochemistry was used to detect the positive expression of Ki-67 and Mucin-4 in the tumors.
The proliferation of ovarian cancer cells was inhibited significantly by C3G with IC50 being 13.82 mg x L(-1). Apoptosis rate induced by C3G was markedly highter than that of control. The expression of Mucin4 was down-regulated in HO-8910PM cells after treatment of C3G. C3G inhibited the growth of ovarian xenograft tumors in nude mice. Furthermore, the positive expression of Ki-67 and Mucin-4 were both decreased in tumors after administration of C3G.
C3G exerts anti-tumor activity in ovarian cancer both in vitro and in vivo, which may be related to down-regulation of Mucin-4 protein.
探讨花青素-3-葡萄糖苷（C3G）在体外和体内对卵巢癌生长抑制的作用及其机制。
用C3G处理人卵巢癌细胞系HO-8910PM后，采用细胞计数试剂盒-8（CCK-8）法测定细胞生长情况，并用Annexin V-FITC/PI染色通过流式细胞术分析评估细胞凋亡。通过蛋白质免疫印迹法分析HO-8910PM细胞中的蛋白质表达。将HO-8910PM细胞皮下注射到裸鼠体内建立异种移植模型。植入3周后，将小鼠随机分为2组（n = 8）：对照组，喂食0.2 mL双蒸水；C3G组，喂食剂量为5 mg·kg⁻¹的C3G。所有处理持续2周，每周3次。植入8周后，处死小鼠，分别评估肿瘤重量和抑制率。采用免疫组织化学法检测肿瘤中Ki-67和粘蛋白-4的阳性表达。
C3G显著抑制卵巢癌细胞的增殖，IC50为13.82 mg·L⁻¹。C3G诱导的凋亡率明显高于对照组。C3G处理后，HO-8910PM细胞中粘蛋白4的表达下调。C3G抑制裸鼠卵巢异种移植肿瘤的生长。此外，给予C3G后，肿瘤中Ki-67和粘蛋白-4的阳性表达均降低。
C3G在体外和体内均对卵巢癌具有抗肿瘤活性，这可能与下调粘蛋白-4蛋白有关。
