Abdallah Fatma M, Hassanin Ola
Department of Virology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Virus Genes. 2013 Feb;46(1):63-70. doi: 10.1007/s11262-012-0821-y. Epub 2012 Sep 21.
Six clinical cases of avipoxvirus (APV) infection were investigated and molecular biologically studied. The samples were collected from different domesticated birds reared in the Egyptian backyard management system and were propagated on the chorioallantoic membrane of embryonated chicken eggs. The virus isolation was confirmed via PCR amplification of fpv167 (P4b) gene locus. All the studied isolates were characterized as Fowlpox-like viruses based on the amplicon length of fpv140 gene locus. The phylogenetic analysis of fpv167 (P4b) gene clustered Elsharqyia_FWPV1, Elsharqyia_FWPV2, Elsharqyia_FWPV3, Elsharqyia_FWPV4, and Elsharqyia_TKPV strains within subclade A1. Furthermore, Elsharqyia_PGPV strain was clustered within subclade A2 (Turkeypox virus) and showed 100 % nucleic acid identity with the wood pigeon Indian which was isolated in 2009. On the other hand, when the fpv140 gene was used for the phylogenetic analysis, Elsharqyia_PGPV was clustered within subclade A4 (Pigeonpox virus) with the other PGPVs. This study is considered the first molecular record for APVs circulating in the Egyptian birds. Further studies in a larger scale need to be developed to have a better understanding about the molecular characterization of the Egyptian APV strains.
对6例禽痘病毒（APV）感染的临床病例进行了调查并开展了分子生物学研究。样本取自埃及后院养殖系统中饲养的不同家禽，并在鸡胚的绒毛尿囊膜上进行繁殖。通过对fpv167（P4b）基因座进行PCR扩增来确认病毒分离情况。根据fpv140基因座的扩增片段长度，所有研究的分离株均被鉴定为禽痘样病毒。对fpv167（P4b）基因的系统发育分析将Elsharqyia_FWPV1、Elsharqyia_FWPV2、Elsharqyia_FWPV3、Elsharqyia_FWPV4和Elsharqyia_TKPV菌株聚类在A1亚分支内。此外，Elsharqyia_PGPV菌株聚类在A2亚分支（火鸡痘病毒）内，与2009年分离的印度林鸽毒株核酸同一性为100%。另一方面，当使用fpv140基因进行系统发育分析时，Elsharqyia_PGPV与其他鸽痘病毒（PGPV）一起聚类在A4亚分支（鸽痘病毒）内。本研究被认为是埃及鸟类中传播的APV的首个分子记录。需要开展更大规模的进一步研究，以更好地了解埃及APV毒株的分子特征。
