Kozdruń Wojciech, Styś-Fijoł Natalia, Czekaj Hanna, Indykiewicz Piotr, Sandecki Rafał, Niczyporuk Jowita Samanta
Department of Poultry Diseases National Veterinary Research Institute, 24-100 Pulawy, Poland.
Faculty of Animal Breeding and Biology University of Sciences and Technology, 85-039 Bydgoszcz, Poland.
J Vet Res. 2018 Dec 31;62(4):427-430. doi: 10.2478/jvetres-2018-0065. eCollection 2018 Dec.
Avian poxvirus infections are widespread in the domestic poultry population but are also reported in wild birds. In poultry, these infections cause significant economic losses, while wild birds may be a reservoir for poxvirus which affects breeding poultry. However, wild birds may also exhibit characteristic anatomopathological changes. This study concerns the infection of wild-living great tits () with the avian poxvirus in Poland.
Samples of internal organs and skin collected from great tits were homogenised and total cellular DNA was isolated. In PCR, the primers complementary to gene encoding the core protein 4b of the HP44 strain of fowl poxvirus (FPV) were used.
After electrophoresis in 2% agarose gel, the PCR product of 578 bp characteristic for FPV was obtained in DNA samples isolated from skin lesions and the heart. The analysis of the nucleotide sequence of the virus strain showed 99% similarity to many poxviruses previously isolated from great tits and other free birds at various sites in the world.
This paper is the first clinically documented evidence obtained in laboratory conditions of avian poxvirus cases in great tits in Poland.
禽痘病毒感染在家禽群体中广泛存在，但在野生鸟类中也有报道。在家禽中，这些感染会造成重大经济损失，而野生鸟类可能是影响种禽的痘病毒的宿主。然而，野生鸟类也可能表现出特征性的解剖病理学变化。本研究关注波兰野生大山雀感染禽痘病毒的情况。
从大山雀采集的内脏器官和皮肤样本进行匀浆处理，并分离出总细胞DNA。在PCR中，使用了与禽痘病毒（FPV）HP44株核心蛋白4b编码基因互补的引物。
在2%琼脂糖凝胶中进行电泳后，从皮肤损伤处和心脏分离的DNA样本中获得了578 bp的FPV特征性PCR产物。对该病毒株核苷酸序列的分析表明，它与之前在世界不同地点从大山雀和其他自由生活鸟类中分离出的许多痘病毒有99%的相似性。
本文是在实验室条件下获得的波兰大山雀禽痘病毒病例的首个临床记录证据。
