Laboratorio de Ecología Microbiana y Biotecnología Marino Tabusso, Dpto de Biología, Universidad Nacional Agraria La Molina, Peru.
Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA (CSIC), Spain.
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1834-1839. doi: 10.1099/ijs.0.042119-0. Epub 2012 Sep 21.
During a study of the 'tunta' (frozen-dry potato) production process in Peru, a bacterial strain, LMT03(T), was isolated from the straw grass in which the potatoes are dried. This strain was classified into the genus Pseudomonas on the basis of the 16S rRNA gene sequence analysis, and is most closely related to Pseudomonas argentinensis CH01(T) with 99.3 % identity in this gene and 96 %, 92 % and 86 % identities in rpoB, rpoD and gyrB genes, respectively. Strain LMT03(T) has a single polar flagellum, like other related yellow-pigment-producing pseudomonads. The major quinone is Q-9. The major fatty acids are C18 : 1ω7c in summed feature 8 (40.82 %), C16 : 1ω6c/C16 : 1ω6c in summed feature 3 (23.72 %) and C16 : 0 (15.20 %). The strain produces oxidase but it does not produce gelatinase, indole, urease, arginine dihydrolase or β-galactosidase. Catalase production was very weak after 28 and 48 h incubation on nutrient agar medium. Nitrate reduction is negative. It does not hydrolyse aesculin. The DNA G+C content is 57.8 mol%. DNA-DNA hybridization results showed lower than 52 % relatedness with respect to the type strain of P. argentinensis, CH01(T). These results, together with other phenotypic characteristics, support the definition of a novel species within the genus Pseudomonas, for which the name Pseudomonas punonensis sp. nov. is proposed. The type strain is LMT03(T) ( = LMG 26839(T) = CECT 8089(T)).
在对秘鲁“tunta”（冻干土豆）生产工艺的研究中，从土豆干燥用的稻草中分离到一株细菌，命名为 LMT03(T)。根据 16S rRNA 基因序列分析，该菌株被归类为假单胞菌属，与 Pseudomonas argentinensis CH01(T)的同源性最高，在该基因上同源性为 99.3%，在 rpoB、rpoD 和 gyrB 基因上的同源性分别为 96%、92%和 86%。菌株 LMT03(T) 具有单极鞭毛，与其他相关的黄色素产生假单胞菌相似。主要醌为 Q-9。主要脂肪酸为 C18 : 1ω7c 在总和特征 8 中（40.82%），C16 : 1ω6c/C16 : 1ω6c 在总和特征 3 中（23.72%）和 C16 : 0（15.20%）。该菌株产生氧化酶，但不产生明胶酶、吲哚、脲酶、精氨酸二氢酶或β-半乳糖苷酶。在营养琼脂培养基上培养 28 和 48 小时后，过氧化氢酶的产生非常微弱。硝酸盐还原为阴性。它不水解马栗树皮苷。DNA G+C 含量为 57.8 mol%。DNA-DNA 杂交结果显示与阿根廷假单胞菌的模式菌株 CH01(T)的相关性低于 52%。这些结果与其他表型特征一起，支持在假单胞菌属内定义一个新种，命名为 Punonensis punonensis sp. nov. 模式菌株为 LMT03(T)（=LMG 26839(T)=CECT 8089(T)）。
