Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA (CSIC).
Instituto de Recursos Naturales y Agrobiología. IRNASA-CSIC, Salamanca, Spain.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3141-3147. doi: 10.1099/ijsem.0.003603. Epub 2019 Jul 23.
During a study on biodiversity of bacteria inhabiting rhizospheric soil of rockrose ( L.), we isolated a strain coded RD25 in a soil from Northern Spain. The 16S rRNA gene sequence showed 99.5 % identity with respect to the closest related species DSM15294, and 99.4 % with respect to WS4672. The following related species showed 99.3 % or less identity, and therefore RD25 was classified within genus . The phylogenetic analysis of 16S rRNA and the housekeeping genes , and suggested that this strain could be a novel species. The strain RD25 has several polar-subpolar flagella. It can grow at 36 °C, at 0-6 % NaCl concentration and a range of pH 5-9. Positive for arginine dihydrolase and urease production, and negative for reduction of nitrate. The strain is catalase and oxidase positive. Major fatty acids are C ω7 / C ω6 in summed feature 3, C, and C ω7 / C ω6 in summed feature 8. The respiratory ubiquinone is Q9. The DNA G+C content was 59.9 mol%. The digital DNA-DNA hybridisation average values (dDDH) ranged between 30-61.2 % relatedness and the ANIb values ranged between 93.9-80.5 % with respect to the type strains of the closely related species. Therefore, the genotypic, genomic, phenotypic and chemotaxonomic data support the classification of strain RD25 as a novel species of genus , for which the name sp. nov. is proposed. The type strain is RD25 (=LMG 30152=CECT 9373).
在一项关于石竹科植物根际土壤中细菌多样性的研究中,我们从西班牙北部的土壤中分离到一株编号为 RD25 的菌株。16S rRNA 基因序列与最接近的相关物种 DSM15294 具有 99.5%的同一性,与 WS4672 具有 99.4%的同一性。以下相关物种的同一性在 99.3%或以下,因此 RD25 被分类为属内的一个新物种。16S rRNA 和看家基因 、 和 的系统发育分析表明,该菌株可能是一个新物种。该菌株 RD25 具有数根极性-亚极性鞭毛。它可以在 36°C、0-6%NaCl 浓度和 pH5-9 的范围内生长。精氨酸二氢酶和脲酶产生阳性,硝酸盐还原阴性。该菌株过氧化氢酶和氧化酶阳性。主要脂肪酸为 C ω7/C ω6 在特征 3 中总和、C 和 C ω7/C ω6 在特征 8 中总和。呼吸醌为 Q9。DNA G+C 含量为 59.9mol%。数字 DNA-DNA 杂交平均数值(dDDH)在 30-61.2%相关度之间,ANIb 值在 93.9-80.5%之间,与密切相关的物种的模式菌株相比。因此,基因型、基因组、表型和化学分类数据支持将菌株 RD25 分类为属的一个新种,提议命名为 sp. nov.。模式菌株为 RD25(=LMG 30152=CECT 9373)。