State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38, Xueyuan Road, Beijing 100191, China.
Nucleic Acids Res. 2012 Nov;40(21):11155-62. doi: 10.1093/nar/gks840. Epub 2012 Sep 22.
Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate β-catenin-2 and no tail expression in zebrafish embryos.
吗啉代寡核苷酸 (MOs) 已被广泛用于敲低斑马鱼中的特定基因，但它们的组成活性限制了它们在研究具有多种功能或在基因网络内的基因的实验应用。本文报道了一种新型的具有两个末端的笼状环状 MO（笼状 cMO）的设计和合成，这两个末端由光可裂解部分连接。这些笼状 cMO 成功地用于光调节斑马鱼胚胎中 β-连环蛋白-2 和无尾的表达。
