Raman J, Hargrave J C
Department of Surgery, St Vincent's Hospital.
Plast Reconstr Surg. 1990 Feb;85(2):248-51. doi: 10.1097/00006534-199002000-00014.
Rehydrated freeze-dried microarterial allografts were implanted to bridge arterial defects using New Zealand White rabbits as the experimental model. Segments of artery from the rabbit ear and thigh were harvested and preserved for a minimum of 2 weeks after freeze-drying. These allografts, approximately 1 mm in diameter and ranging from 1.5 to 2.5 cm in length, were rehydrated and then implanted in low-pressure and high-pressure arterial systems. Poor patency was noted in low-pressure systems in both allografts and autografts, tested in 12 rabbits. In the high-pressure arterial systems, allografts that were freeze-dried and reconstituted failed in a group of 10 rabbits with an 8-week patency rate of 30 percent. Gamma irradiation in an effort to reduce infection and antigenicity of grafts after freeze-drying was associated with a patency rate of 10 percent at 8 weeks in this system in another group of 10 rabbits. Postoperative cyclosporin A therapy was associated with a patency rate of 22.2 percent in the high-pressure arterial system in a 9-rabbit group. Control autografts in this system in a group of 10 rabbits showed a 100 percent patency at 8 weeks. Microarterial grafts depend on perfusion pressure of the vascular bed for long-term patency. Rehydrated freeze-dried microarterial allografts do not seem to function well in lengths of 1 to 2.5 cm when implanted in a high-pressure arterial system. Freeze-dried arterial allografts are probably not antigenic.
以新西兰白兔为实验模型，将复水冻干的同种异体微动脉移植片植入以桥接动脉缺损。采集兔耳和大腿的动脉段，冻干后至少保存2周。这些直径约1mm、长度在1.5至2.5cm之间的移植片复水后，植入低压和高压动脉系统。在对12只兔子进行测试时，发现同种异体移植片和自体移植片在低压系统中的通畅性均较差。在高压动脉系统中，一组10只兔子中冻干并复水的同种异体移植片失败，8周通畅率为30%。在另一组10只兔子中，为降低冻干后移植片的感染性和抗原性而进行的γ射线照射，在该系统中8周时的通畅率为10%。术后环孢素A治疗在一组9只兔子的高压动脉系统中的通畅率为22.2%。一组10只兔子在该系统中的对照自体移植片在8周时显示100%的通畅率。微动脉移植片的长期通畅依赖于血管床的灌注压力。复水冻干的同种异体微动脉移植片在植入高压动脉系统时，长度在1至2.5cm之间似乎功能不佳。冻干的动脉同种异体移植片可能没有抗原性。
