Pratt M F, Schneider J R, Galey F R
Arch Otolaryngol Head Neck Surg. 1987 Sep;113(9):953-8. doi: 10.1001/archotol.1987.01860090051018.
Arterial allografts, 3.5 cm in length, were freeze-dried and placed into the femoral arteries of 20 female rabbits (16 experimental subjects and four controls). Immediate patency was 100%. Subjects were surgically explored after two months of observation. Overall patency at this time was 31% (5/16). A patency rate of 50% (5/10) was achieved with size-matched femoral grafts. However, all of the smaller diameter brachial grafts were unsuccessful. Analysis by light microscopy as well as transmission and scanning electron microscopy demonstrated intimal hyperplasia, which was more prominent in nonpatent grafts. There was no evidence of a cellular immune response to the freeze-dried grafts by the host. The use of size-matched grafts and postoperative anticoagulants in future studies may improve patency rates and the potential clinical applicability of this promising microvascular technique.
长度为3.5厘米的动脉同种异体移植物经冻干后植入20只雌性兔子（16只实验对象和4只对照）的股动脉中。即时通畅率为100%。观察两个月后对实验对象进行手术探查。此时的总体通畅率为31%（5/16）。尺寸匹配的股动脉移植物通畅率达到50%（5/10）。然而，所有直径较小的肱动脉移植物均未成功。通过光学显微镜以及透射和扫描电子显微镜分析显示存在内膜增生，在未通畅的移植物中更为明显。没有证据表明宿主对冻干移植物产生细胞免疫反应。在未来的研究中使用尺寸匹配的移植物和术后抗凝剂可能会提高通畅率以及这种有前景的微血管技术的潜在临床适用性。
