可食用蓝细菌念珠藻属中类咕啉化合物的表征

Characterization of corrinoid compounds from edible cyanobacterium Nostochopsis sp.

作者信息

Hashimoto Eri, Yabuta Yukinori, Takenaka Shigeo, Yamaguchi Yuji, Takenaka Hiroyuki, Watanabe Fumio

机构信息

School of Agricultural, Biological and Environmental Sciences, Tottori University, Tottori, Japan.

出版信息

J Nutr Sci Vitaminol (Tokyo). 2012;58(1):50-3. doi: 10.3177/jnsv.58.50.

DOI:10.3177/jnsv.58.50

PMID:23007067

Abstract

Vitamin B₁₂ content of an edible cyanobacterium, Nostochopsis sp. was determined to be 140.6±16.2 μg/100 g dry weight by a microbiological method. To evaluate whether the Nostochopsis cells contain vitamin B₁₂ or inactive corrinoid compounds, corrinoid compounds were purified from the cells and then identified as pseudovitamin B₁₂ (97.4±11.8 μg/100 g dry weight) and vitamin B₁₂ (43.2±6.0 μg/100 g dry weight) on the basis of silica gel 60 TLC bioautograms and LC/ESI-MS/MS chromatograms. Vitamin B₁₂ content was significantly increased in the Nostochopsis cells (254.8±17.6 μg/100 g dry weight) grown in the vitamin B₁₂-supplemented medium.

摘要

通过微生物学方法测定，可食用蓝细菌Nostochopsis sp.的维生素B₁₂含量为140.6±16.2μg/100g干重。为评估Nostochopsis细胞中是否含有维生素B₁₂或无活性的类咕啉化合物，从细胞中纯化出类咕啉化合物，然后根据硅胶60 TLC生物自显影图谱和LC/ESI-MS/MS色谱图将其鉴定为假维生素B₁₂（97.4±11.8μg/100g干重）和维生素B₁₂（43.2±6.0μg/100g干重）。在添加了维生素B₁₂的培养基中生长的Nostochopsis细胞中，维生素B₁₂含量显著增加（254.8±17.6μg/100g干重）。