Raymundo T M, Favilla M, Niero R, Andrade S F, Maistro E L
Departamento de Fonoaudiologia, Faculdade de Filosofia e Ciências, Universidade Estadual Paulista "Julio de Mesquita Filho", Marília, SP, Brasil.
Genet Mol Res. 2012 Aug 24;11(3):2847-54. doi: 10.4238/2012.August.24.9.
Plants belonging to the Celastraceae family have been used in traditional medicine for their analgesic, anti-inflammatory and anti-ulcerogenic properties, among others. Maytenus ilicifolia is the principal species of this family, and is used in the treatment of gastric ulcers. However, owing to its inadequate management in Brazil, the species is becoming extinct and is being substituted with Maytenus robusta, which also displays gastroprotective activity. The aim of this study was to evaluate the genotoxic effects of M. robusta hydroalcoholic extract in vivo, using the comet assay and micronucleus test. Three doses (50, 250 and 500 mg/kg body weight) were administered to mice orally 2 times at 24-h intervals. Cytotoxicity was assessed by scoring 200 consecutive total polychromatic and normochromatic erythrocytes to calculate their ratio. Parametric (analysis of variance/Tukey) and non-parametric (Kruskal-Wallis/Dunn post hoc) tests were used to evaluate the results according to the nature of the data distribution. The results showed a significant increase in the frequency of DNA damage on leukocytes at the 2 higher doses tested, but the extract did not enhance micronucleus frequency in bone marrow cells. Our findings showed that after 48 h of treatment, M. robusta hydroalcoholic extract had weak genotoxic effects but no clastogenic effects in mice cells.
卫矛科植物因其具有止痛、抗炎和抗溃疡等特性而被用于传统医学。冬青叶美登木是该科的主要物种，用于治疗胃溃疡。然而，由于其在巴西管理不善，该物种正在灭绝，并被同样具有胃保护活性的粗壮美登木所取代。本研究的目的是使用彗星试验和微核试验评估粗壮美登木水醇提取物在体内的遗传毒性作用。以24小时间隔给小鼠口服三种剂量（50、250和500毫克/千克体重），共给药2次。通过对200个连续的多色和正色红细胞进行评分以计算其比例来评估细胞毒性。根据数据分布的性质，使用参数检验（方差分析/ Tukey检验）和非参数检验（Kruskal-Wallis检验/ Dunn事后检验）来评估结果。结果显示，在测试的2个较高剂量下，白细胞DNA损伤频率显著增加，但提取物并未提高骨髓细胞中的微核频率。我们的研究结果表明，在治疗48小时后，粗壮美登木水醇提取物对小鼠细胞具有较弱的遗传毒性作用，但没有断裂作用。
