Department of Pathology, Georgetown University Medical Center, Washington, DC 20057, USA.
N Engl J Med. 2012 Sep 27;367(13):1220-7. doi: 10.1056/NEJMoa1203055.
A patient with a 20-year history of recurrent respiratory papillomatosis had progressive, bilateral tumor invasion of the lung parenchyma. We used conditional reprogramming to generate cell cultures from the patient's normal and tumorous lung tissue. Analysis revealed that the laryngeal tumor cells contained a wild-type 7.9-kb human papillomavirus virus type 11 (HPV-11) genome, whereas the pulmonary tumor cells contained a 10.4-kb genome. The increased size of the latter viral genome was due to duplication of the promoter and oncogene regions. Chemosensitivity testing identified vorinostat as a potential therapeutic agent. At 3 months after treatment initiation, tumor sizes had stabilized, with durable effects at 15 months.
一位患有 20 年复发性呼吸道乳头瘤病的患者，其肺部实质出现进行性双侧肿瘤浸润。我们使用条件重编程，从患者的正常和肿瘤肺组织中生成细胞培养物。分析显示，喉肿瘤细胞含有野生型 7.9kb 人乳头瘤病毒 11 型（HPV-11）基因组，而肺肿瘤细胞则含有 10.4kb 基因组。后者病毒基因组的增大是由于启动子和癌基因区域的重复。化疗敏感性测试确定伏立诺他为一种潜在的治疗药物。在开始治疗 3 个月后，肿瘤大小稳定，15 个月时仍有持久效果。
