Cover Timothy L
Department of Medicine and Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.
Methods Mol Biol. 2012;921:11-5. doi: 10.1007/978-1-62703-005-2_3.
Over the past 25 years, a variety of methods have been developed for culture of Helicobacter pylori in vitro. H. pylori is a capnophilic and microaerophilic organism that is typically cultured using complex culture media. Analysis of H. pylori growth in chemically defined media has provided insight into the nutritional requirements, physiology, and metabolic capacities of this organism.
在过去25年里,已开发出多种用于幽门螺杆菌体外培养的方法。幽门螺杆菌是一种嗜二氧化碳和微需氧的微生物,通常使用复杂培养基进行培养。对幽门螺杆菌在化学成分明确的培养基中生长情况的分析,为了解该微生物的营养需求、生理学和代谢能力提供了线索。