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Unique host iron utilization mechanisms of Helicobacter pylori revealed with iron-deficient chemically defined media.幽门螺杆菌利用缺铁化学定义培养基揭示其独特的宿主铁利用机制。
Infect Immun. 2010 May;78(5):1841-9. doi: 10.1128/IAI.01258-09. Epub 2010 Feb 22.
2
The effect of the human gut-signalling hormone, norepinephrine, on the growth of the gastric pathogen Helicobacter pylori.人体肠道信号激素去甲肾上腺素对胃部病原体幽门螺杆菌生长的影响。
Helicobacter. 2009 Jun;14(3):223-30. doi: 10.1111/j.1523-5378.2009.00682.x.
3
Roles of alpha and beta carbonic anhydrases of Helicobacter pylori in the urease-dependent response to acidity and in colonization of the murine gastric mucosa.幽门螺杆菌的α和β碳酸酐酶在脲酶依赖性酸度反应及小鼠胃黏膜定植中的作用
Infect Immun. 2008 Feb;76(2):497-509. doi: 10.1128/IAI.00993-07. Epub 2007 Nov 19.
4
Direct analysis of the extracellular proteome from two strains of Helicobacter pylori.对两株幽门螺杆菌细胞外蛋白质组的直接分析。
Proteomics. 2007 Jun;7(13):2240-5. doi: 10.1002/pmic.200600875.
5
Flavodoxin:quinone reductase (FqrB): a redox partner of pyruvate:ferredoxin oxidoreductase that reversibly couples pyruvate oxidation to NADPH production in Helicobacter pylori and Campylobacter jejuni.黄素氧还蛋白：醌还原酶（FqrB）：丙酮酸：铁氧化还原蛋白氧化还原酶的一种氧化还原伴侣，在幽门螺杆菌和空肠弯曲菌中，它可将丙酮酸氧化与NADPH生成可逆偶联。
J Bacteriol. 2007 Jul;189(13):4764-73. doi: 10.1128/JB.00287-07. Epub 2007 Apr 27.
6
Peptide transport in Helicobacter pylori: roles of dpp and opp systems and evidence for additional peptide transporters.幽门螺杆菌中的肽转运：二肽转运蛋白（dpp）和寡肽转运蛋白（opp）系统的作用以及其他肽转运体的证据
J Bacteriol. 2007 May;189(9):3392-402. doi: 10.1128/JB.01636-06. Epub 2007 Feb 23.
7
Is Helicobacter pylori a true microaerophile?幽门螺杆菌是真正的微需氧菌吗？
Helicobacter. 2006 Aug;11(4):296-303. doi: 10.1111/j.1523-5378.2006.00413.x.
8
Nutritional requirements and antibiotic resistance patterns of Helicobacter species in chemically defined media.化学限定培养基中幽门螺杆菌的营养需求和抗生素耐药模式。
J Clin Microbiol. 2006 May;44(5):1650-8. doi: 10.1128/JCM.44.5.1650-1658.2006.
9
Culturing Helicobacter pylori from clinical specimens: review of microbiologic methods.
J Pediatr Gastroenterol Nutr. 2003 May;36(5):616-22. doi: 10.1097/00005176-200305000-00005.
10
Identification and characterization of Helicobacter pylori genes essential for gastric colonization.幽门螺杆菌在胃中定殖所必需基因的鉴定与表征
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幽门螺杆菌体外培养方法的研究视角

Perspectives on methodology for in vitro culture of Helicobacter pylori.

作者信息

Cover Timothy L

机构信息

Department of Medicine and Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, USA.

出版信息

Methods Mol Biol. 2012;921:11-5. doi: 10.1007/978-1-62703-005-2_3.

DOI:10.1007/978-1-62703-005-2_3
PMID:23015486
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3921885/
Abstract

Over the past 25 years, a variety of methods have been developed for culture of Helicobacter pylori in vitro. H. pylori is a capnophilic and microaerophilic organism that is typically cultured using complex culture media. Analysis of H. pylori growth in chemically defined media has provided insight into the nutritional requirements, physiology, and metabolic capacities of this organism.

摘要

在过去25年里，已开发出多种用于幽门螺杆菌体外培养的方法。幽门螺杆菌是一种嗜二氧化碳和微需氧的微生物，通常使用复杂培养基进行培养。对幽门螺杆菌在化学成分明确的培养基中生长情况的分析，为了解该微生物的营养需求、生理学和代谢能力提供了线索。