Wang Dan, Nambu Takayuki, Tanimoto Hiroaki, Iwata Naohiro, Yoshikawa Kazushi, Okinaga Toshinori, Yamamoto Kazuyo
Department of Operative Dentistry, Graduate School of Dentistry, Osaka Dental University, 8-1, Kuzuha-Hanazono, Hirakata, Osaka 573-1121, Japan.
Department of Bacteriology, Osaka Dental University, 8-1, Kuzuha-Hanazono, Hirakata, Osaka 573-1121, Japan.
Antibiotics (Basel). 2021 Nov 4;10(11):1348. doi: 10.3390/antibiotics10111348.
Oral microbiome dysbiosis has important links to human health and disease. Although photodynamic therapy influences microbiome diversity, the specific effect of violet light irradiation remains largely unknown. In this study, we analyzed the effect of violet light-emitting diode (LED) irradiation on interdental plaque microbiota. Interdental plaque was collected from 12 human subjects, exposed to violet LED irradiation, and cultured in a specialized growth medium. Next-generation sequencing of the 16S ribosomal RNA genes revealed that α-diversity decreased, whereas β-diversity exhibited a continuous change with violet LED irradiation doses. In addition, we identified several operational taxonomic units that exhibited significant shifts during violet LED irradiation. Specifically, violet LED irradiation led to a significant reduction in the relative abundance of species, but a significant increase in several species of oral bacteria, such as and . Our study provides an overview of oral plaque microbiota changes under violet LED irradiation, and highlights the potential of this method for adjusting the balance of the oral microbiome without inducing antibiotic resistance.
口腔微生物群失调与人类健康和疾病有着重要联系。尽管光动力疗法会影响微生物群多样性，但紫光照射的具体效果仍 largely unknown。在本研究中，我们分析了紫光发光二极管（LED）照射对牙菌斑微生物群的影响。从12名人类受试者中收集牙菌斑，使其暴露于紫光LED照射下，并在专门的生长培养基中培养。对16S核糖体RNA基因进行的下一代测序显示，α多样性降低，而β多样性随着紫光LED照射剂量呈现持续变化。此外，我们鉴定出了几个在紫光LED照射期间表现出显著变化的操作分类单元。具体而言，紫光LED照射导致 物种的相对丰度显著降低，但几种口腔细菌的物种显著增加，如 和 。我们的研究概述了紫光LED照射下口腔菌斑微生物群的变化，并突出了该方法在不诱导抗生素耐药性的情况下调节口腔微生物群平衡的潜力。
