对自然感受态细菌幽门螺杆菌进行基因操作。
Genetic manipulation of a naturally competent bacterium, Helicobacter pylori.
作者信息
Noto Jennifer M, Peek Richard M
机构信息
Division of Gastroenterology, Department of Medicine, Vanderbilt University Medical Center, MRB IV 1030C MRB IV, Nashville, TN, USA.
出版信息
Methods Mol Biol. 2012;921:51-9. doi: 10.1007/978-1-62703-005-2_8.
Abstract
Genetic manipulation of Helicobacter pylori facilitates characterization and functional analysis of individual H. pylori genes. This chapter discusses the methods involved in H. pylori chromosomal DNA isolation, mutagenesis of individual genes, and natural transformation.
摘要
幽门螺杆菌的基因操作有助于对单个幽门螺杆菌基因进行表征和功能分析。本章讨论了幽门螺杆菌染色体DNA分离、单个基因突变以及自然转化所涉及的方法。
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