Morales Adelaida, Espino Ana M
Laboratory of Immunology and Molecular Parasitology, Department of Microbiology, University of Puerto Rico, School of Medicine, San Juan, Puerto Rico.
Clin Vaccine Immunol. 2012 Nov;19(11):1870-8. doi: 10.1128/CVI.00487-12. Epub 2012 Sep 26.
Tegument protein extract from Fasciola hepatica adult flukes (FhTA) was obtained and assessed for its potential as a diagnostic agent for the serological detection of human fascioliasis using an indirect enzyme-linked immunosorbent assay (ELISA). In an analysis of sera from 45 patients infected with F. hepatica, sera from 41 patients with other parasitic infections, and sera from 33 healthy controls, the FhTA-ELISA showed sensitivity, specificity, and accuracy of 91.1%, 97.3%, and 95%, respectively. Specific IgG1 and IgG4 were the antibody isotypes mainly detected in sera from patients with fascioliasis. Polypeptides of 52, 38, 24 to 26, and 12 to 14 kDa were identified by Western blotting as the most immunoreactive components of the FhTA. A proteomic approach led us to identify enolase, aldolase, glutathione S-transferase, and fatty acid binding protein as the major immunoreactive components of the FhTA.
从肝片吸虫成虫中获取了皮层蛋白提取物（FhTA），并使用间接酶联免疫吸附测定（ELISA）评估其作为人肝片吸虫病血清学检测诊断试剂的潜力。在对45例感染肝片吸虫的患者血清、41例患有其他寄生虫感染的患者血清以及33例健康对照血清的分析中，FhTA-ELISA的敏感性、特异性和准确性分别为91.1%、97.3%和95%。特异性IgG1和IgG4是在肝片吸虫病患者血清中主要检测到的抗体亚型。通过蛋白质印迹法鉴定出52、38、24至26以及12至14 kDa的多肽是FhTA中免疫反应性最强的成分。蛋白质组学方法使我们鉴定出烯醇化酶、醛缩酶、谷胱甘肽S-转移酶和脂肪酸结合蛋白是FhTA的主要免疫反应性成分。
