Bakris G L, Lass N, Gaber A O, Jones J D, Burnett J C
Department of Physiology, Mayo Graduate School of Medicine, Rochester, Minnesota 55905.
Am J Physiol. 1990 Jan;258(1 Pt 2):F115-20. doi: 10.1152/ajprenal.1990.258.1.F115.
Intrarenal injection of radiocontrast medium (RCM) results in transient vasoconstriction and a persistent decline in glomerular filtration rate (GFR). Adenosine modulates this vasoconstrictor response and is postulated to increase oxygen free radical (OFR) generation. We hypothesized that the persistent decline in (GFR that follows RCM administration results in an increased generation of OFR. We evaluated the effects of RCM injection on renal blood flow, inulin clearance, hypoxanthine, xanthine, and malondialdehyde concentrations in four groups of non-volume-expanded, pentobarbital sodium anesthetized dogs in the presence and absence of intravenous allopurinol, 25 mg/min (group 1), intrarenal superoxide dismutase (SOD), 400 U/min (group 2), heat-inactivated intrarenal SOD, 400 U/min (group 3), and simultaneous infusions of intrarenal SOD, 400 U/min, to one kidney and saline to the other (group 4). Both allopurinol and SOD significantly attenuated the fall in GFR after RCM administration over control. Malondialdehyde concentrations were attenuated over control in all treated groups, indicating a decrease in OFR generation. We conclude that intrarenal injection of RCM results in increased production of OFR. Inhibition of OFR production by allopurinol and increased OFR removal by SOD attenuates the effects of RCM on declines in GFR.
肾内注射放射性造影剂（RCM）会导致短暂的血管收缩以及肾小球滤过率（GFR）持续下降。腺苷可调节这种血管收缩反应，并被认为会增加氧自由基（OFR）的生成。我们假设，RCM给药后GFR的持续下降会导致OFR生成增加。我们评估了在有和没有静脉注射别嘌呤醇（25毫克/分钟，第1组）、肾内超氧化物歧化酶（SOD，400单位/分钟，第2组）、热灭活肾内SOD（400单位/分钟，第3组）以及同时向一侧肾脏输注肾内SOD（400单位/分钟）而向另一侧肾脏输注生理盐水（第4组）的情况下，RCM注射对四组非容量扩张、戊巴比妥钠麻醉犬的肾血流量、菊粉清除率、次黄嘌呤、黄嘌呤和丙二醛浓度的影响。与对照组相比，别嘌呤醇和SOD均显著减轻了RCM给药后GFR的下降。所有治疗组的丙二醛浓度均低于对照组，表明OFR生成减少。我们得出结论，肾内注射RCM会导致OFR生成增加。别嘌呤醇对OFR生成的抑制以及SOD对OFR清除的增加减弱了RCM对GFR下降的影响。
