Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, P, R, China.
Lipids Health Dis. 2012 Sep 28;11:124. doi: 10.1186/1476-511X-11-124.
The hydrolysis activities of three alkaline lipases, L-A1, L-A2 and L-A3 secreted by different lipase-producing microorganisms isolated from the Bay of Bohai, P. R. China were characterized with 16 kinds of esters. It was found that all the lipases have the ability to catalyze the hydrolysis of the glycerides, methyl esters, ethyl esters, especially for triglycerides, which shows that they have broad substrate spectra, and this property is very important for them to be used in detergent industry. Three QSAR models were built for L-A1, L-A2 and L-A3 respectively with GFA using Discovery studio 2.1. The models equations 1, 2 and 3 can explain 95.80%, 97.45% and 97.09% of the variances (R(2)(adj)) respectively while they could predict 95.44%, 89.61% and 93.41% of the variances (R(2)(cv)) respectively. With these models the hydrolysis activities of these lipases to mixed esters were predicted and the result showed that the predicted values are in good agreement with the measured values, which indicates that this method can be used as a simple tool to predict the lipase activities for single or mixed esters.
从中国渤海湾分离得到的三株产碱性脂肪酶的微生物所分泌的 3 种脂肪酶（L-A1、L-A2 和 L-A3）具有水解 16 种酯的活性。结果发现所有的脂肪酶都能够催化甘油酯、甲酯和乙酯的水解，尤其是对三酸甘油酯的水解，这表明它们具有广泛的底物谱，这一特性对于它们在洗涤剂工业中的应用非常重要。用 Discovery studio 2.1 中的 GFA 分别为 L-A1、L-A2 和 L-A3 建立了 3 个 QSAR 模型。模型方程 1、2 和 3 分别可以解释 95.80%、97.45%和 97.09%的方差（R(2)(adj)），而它们可以预测 95.44%、89.61%和 93.41%的方差（R(2)(cv)）。利用这些模型对混合酯的水解活性进行了预测，结果表明预测值与实测值吻合较好，这表明该方法可作为一种简单的工具来预测脂肪酶对单一或混合酯的水解活性。
