Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology , Tianjin 300457 , China.
Braz J Microbiol. 2012 Jan;43(1):148-56. doi: 10.1590/S1517-838220120001000016. Epub 2012 Jun 1.
A novel alkaline lipase-producing strain 1-7 identified as Acinetobacter calcoaceticus was isolated from soil samples collected from Bohai Bay, China, using an olive oil alkaline plate, which contained olive oil as the sole carbon source. The lipase from strain 1-7 showed the maximum activity at pH 9.0 under 40 °C. One interesting feature of this enzyme is that it exhibits lipase activity over a broad range of temperatures and good stability. It is also stable at a broad range of pHs from 4.0 to 10.0 for 24 h. Its catalytic activity was highly enhanced in the presence of Ca(2+), Mg(2+) and K(+), but partially inhibited by Cu(2+), Al(3+), Fe(3+), Ba(2+)and Zn(2+). The fact that it displays marked stability and activity in the presence of TritonX-100, Tween-20, Tween-80, SDS, Hydrogen peroxide, Sodium perborate, Sodium hypochlorite, Sodium citrate, Sodium taurocholate, Glycerine and NaCl suggests that this lipase is suitable as an additive in detergent formulations.
从中国渤海湾采集的土壤样本中，利用橄榄油碱性平板（以橄榄油为唯一碳源）分离出一株产碱性脂肪酶的新菌株 1-7，鉴定为醋酸钙不动杆菌。菌株 1-7 产生的脂肪酶在 40°C 下 pH9.0 时活性最高。该酶的一个有趣特点是在较宽的温度范围内具有脂肪酶活性，且稳定性良好。它在 pH4.0 到 10.0 之间的宽范围内保持 24 小时的稳定性。在 Ca(2+)、Mg(2+)和 K(+)的存在下，其催化活性显著增强，但 Cu(2+)、Al(3+)、Fe(3+)、Ba(2+)和 Zn(2+)部分抑制其活性。在 TritonX-100、Tween-20、Tween-80、SDS、H2O2、过硼酸钠、次氯酸钠、柠檬酸钠、牛磺胆酸钠、甘油和 NaCl 存在下，该脂肪酶显示出明显的稳定性和活性，表明该脂肪酶适合作为洗涤剂配方中的添加剂。
