Saisubramanian N, Edwinoliver N G, Nandakumar N, Kamini N R, Puvanakrishnan R
Department of Biotechnology, Central Leather Research Institute, 600 020, Adyar, Chennai, India,
J Ind Microbiol Biotechnol. 2006 Aug;33(8):669-76. doi: 10.1007/s10295-006-0100-9. Epub 2006 Feb 21.
The efficacy of lipase from Aspergillus niger MTCC 2594 as an additive in laundry detergent formulations was assessed using response surface methodology (RSM). A five-level four-factorial central composite design was chosen to explain the washing protocol with four critical factors, viz. detergent concentration, lipase concentration, buffer pH and washing temperature. The model suggested that all the factors chosen had a significant impact on oil removal and the optimal conditions for the removal of olive oil from cotton fabric were 1.0% detergent, 75 U of lipase, buffer pH of 9.5 and washing temperature of 25 degrees C. Under optimal conditions, the removal of olive oil from cotton fabric was 33 and 17.1% at 25 and 49 degrees C, respectively, in the presence of lipase over treatment with detergent alone. Hence, lipase from A. niger could be effectively used as an additive in detergent formulation for the removal of triglyceride soil both in cold and warm wash conditions.
采用响应面法（RSM）评估了黑曲霉MTCC 2594脂肪酶作为洗衣粉配方添加剂的效果。选择了五水平四因素中心复合设计来解释洗涤方案，其中有四个关键因素，即洗涤剂浓度、脂肪酶浓度、缓冲液pH值和洗涤温度。该模型表明，所选的所有因素对油污去除都有显著影响，从棉织物上去除橄榄油的最佳条件是1.0%的洗涤剂、75 U的脂肪酶、缓冲液pH值为9.5以及洗涤温度为25℃。在最佳条件下，与仅用洗涤剂处理相比，在有脂肪酶存在的情况下，25℃和49℃时从棉织物上去除橄榄油的比例分别为33%和17.1%。因此，黑曲霉脂肪酶可有效地用作洗涤剂配方中的添加剂，用于在冷洗和温洗条件下去除甘油三酯污垢。
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