Samba Nsevolo, Barrios Abdy Morales, De León Estela Guerrero, Raposo Cesar, Lahlou Radhia Aitfella, Curto Joana, Rodilla Jesus M, Roncero Alejandro M, Diez David, Silva Lúcia
Chemistry Department, University of Beira Interior, 6201-001 Covilhã, Portugal.
Department of Clinical Analysis and Public Health, University Kimpa Vita, Uige 77, Angola.
Molecules. 2025 Jun 27;30(13):2768. doi: 10.3390/molecules30132768.
The phytochemical investigation and evaluation of the antioxidant activity of the leaves, bark, and roots of Welw ex Oliv-a valued plant that is widely used in traditional Angolan medicine-hold significant importance. Compounds were extracted from the aforementioned plant using acetone and ethanol and identified by HPLC-ESI-MSn. Both extracts demonstrated notable abilities to scavenge 2,2-diphenyl-1-picrylhydrazyl, nitric oxide, and superoxide radicals, as well as to inhibit lipid peroxidation. A HPLC analysis revealed a diverse array of bioactive compounds, including flavonoids, phenols, alkaloids, quinones, and terpenes, which help neutralize free radicals and protect cells against oxidative stress, thereby contributing to the prevention of various diseases. Moreover, the acetone and ethanol extracts proved to be excellent sources of antioxidants. For the first time, the present study identified new compounds never reported in this species, such as (+)-abscisic acid, angustine B, pinobanksin, dihydrogenistein, (-)-8-prenylnaringenin, isoquercetin, samandarine, dihydromyricetin, and eupatoriocromene, in the leaves, bark, and roots, marking a significant advance in the chemical characterization of . These findings enhance our understanding of the bioactive phytochemicals and antioxidant properties of and open new avenues for future therapeutic and pharmacological research, further supporting its traditional use in Angolan medicine.
对橄榄叶、树皮和根进行植物化学研究并评估其抗氧化活性具有重要意义。橄榄是一种珍贵的植物，在安哥拉传统医学中广泛使用。使用丙酮和乙醇从上述植物中提取化合物，并通过HPLC-ESI-MSn进行鉴定。两种提取物均表现出显著的清除2,2-二苯基-1-苦基肼、一氧化氮和超氧阴离子自由基的能力，以及抑制脂质过氧化的能力。HPLC分析显示出多种生物活性化合物，包括黄酮类、酚类、生物碱、醌类和萜类，这些化合物有助于中和自由基并保护细胞免受氧化应激，从而有助于预防各种疾病。此外，丙酮和乙醇提取物被证明是抗氧化剂的优质来源。本研究首次在该物种的叶、树皮和根中鉴定出从未报道过的新化合物，如（+）-脱落酸、狭叶番荔枝碱B、皮诺蒎醛、二氢大豆素、（-）-8-异戊烯基柚皮素、异槲皮素、萨曼达林、二氢杨梅素和泽兰内酯，这标志着在该植物的化学表征方面取得了重大进展。这些发现增进了我们对该植物生物活性植物化学物质和抗氧化特性的理解，并为未来的治疗和药理学研究开辟了新途径，进一步支持了其在安哥拉医学中的传统用途。
