Key Laboratory of Crop Physiology, Ecology and Genetic Breeding-Jiangxi Agricultural University, Ministry of Education, Jiangxi Province 330045, China.
Plant Sci. 2012 Nov;196:32-43. doi: 10.1016/j.plantsci.2012.07.013. Epub 2012 Aug 8.
The rise of global warming presents a problem for all living organisms, including rice and other staple plants. High temperatures impair rice grain weight by inhibiting the filling of the caryopses during the milky stage. The molecular mechanism behind this process, however, is poorly understood. Identifying candidate genes involved in responses to high-temperature stress may provide a basis for the improvement of heat tolerance in rice. Using paired, genetically similar heat-tolerant and heat-sensitive rice lines as plant materials, cDNA-AFLP analysis revealed a total of 54 transcript derived fragments (TDFs), mainly from the heat-tolerant lines. This clearly indicated variations in gene expression between the two rice lines. BLAST results showed that 28 of the 54 TDFs were homologous sequences. These homologous genes were found to encode proteins involved in signal transduction, oxidation, transcriptional regulation, transport, and metabolism. The functions and differential expression patterns of some important genes are further discussed. High temperature stress may trigger a wide range of changes in gene expression in rice caryopses, in turn affecting functions ranging from signal transduction to cellular metabolism. Forty-five of the 54 TDFs were mapped to rice chromosomes. The genes identified in the present study would make good candidates for further study into the molecular mechanisms underlying rice adaptation to high-temperature stress.
全球变暖的加剧给包括水稻和其他主要农作物在内的所有生物都带来了问题。高温通过抑制乳熟期稻谷的灌浆来降低稻谷的重量。然而，这一过程的分子机制还不太清楚。鉴定参与高温胁迫响应的候选基因，可能为提高水稻的耐热性提供基础。利用配对的、遗传上相似的耐热和热敏水稻品系作为植物材料，cDNA-AFLP 分析共揭示了 54 个转录衍生片段（TDFs），主要来自耐热品系。这清楚地表明了两个水稻品系之间的基因表达存在差异。BLAST 结果表明，54 个 TDF 中有 28 个是同源序列。这些同源基因编码的蛋白参与信号转导、氧化、转录调控、运输和代谢。进一步讨论了一些重要基因的功能和差异表达模式。高温胁迫可能会引发水稻灌浆过程中广泛的基因表达变化，从而影响从信号转导到细胞代谢的各种功能。54 个 TDF 中有 45 个被定位到水稻染色体上。本研究中鉴定的基因可能是进一步研究水稻适应高温胁迫的分子机制的良好候选基因。
