Rodríguez Mayra, Canales Eduardo, Borroto Carlos J, Carmona Elva, López Junior, Pujol Merardo, Borrás-Hidalgo Orlando
Laboratory of Plant Functional Genomics, Head of the Plant Functional Genomic Department, Plant Division, Center for Genetic Engineering and Biotechnology (C.I.G.B.), P.O. Box 6162, La Habana 10600, Cuba.
J Plant Physiol. 2006 Mar;163(5):577-84. doi: 10.1016/j.jplph.2005.07.005. Epub 2005 Sep 12.
Among the abiotic stresses, the availability of water is the most important factor that limits the productive potential of higher plants. The identification of novel genes, determination of their expression patterns, and the understanding of their functions in stress adaptation is essential to improve stress tolerance. Amplified fragment length polymorphism analysis of cDNA was used to identify rice genes differentially expressed in a tolerant rice variety upon water-deficit stress. In total, 103 transcript-derived fragments corresponding to differentially induced genes were identified. The results of the sequence comparison in BLAST database revealed that several differentially expressed TDFs were significantly homologous to stress regulated genes/proteins isolated from rice or other plant species. Most of the transcripts identified here were genes related to metabolism, energy, protein biosynthesis, cell defence, signal transduction, and transport. New genes involved in the response to water-deficit stress in a tolerant rice variety are reported here.
在非生物胁迫中，水分可利用性是限制高等植物生产潜力的最重要因素。鉴定新基因、确定其表达模式以及了解其在胁迫适应中的功能对于提高胁迫耐受性至关重要。利用cDNA的扩增片段长度多态性分析来鉴定在水分亏缺胁迫下耐水稻品种中差异表达的水稻基因。总共鉴定出103个与差异诱导基因相对应的转录本衍生片段。在BLAST数据库中的序列比较结果表明，几个差异表达的TDF与从水稻或其他植物物种中分离出的胁迫调节基因/蛋白质具有显著同源性。这里鉴定出的大多数转录本是与代谢、能量、蛋白质生物合成、细胞防御、信号转导和运输相关的基因。本文报道了耐水稻品种中参与水分亏缺胁迫响应的新基因。
